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179,844 results

Showing: 9181 - 9200 of 179844 results

  3. Gi2-CASE

    Plasmid
    #168121
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi2. Composed of the subunits G alpha i2 (GNAI2) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).
    Depositor
    Gunnar Schulte
    Publication
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNAI2, GNB1, GNG2 (GNAI2, GNB1, GNG2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG2
    Expression
    Mammalian
    Mutation
    NLuc is inserted at C112/E115 within GNAI2
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    168121-plasmid-map-sequence-id-332861

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  4. pRDA_221

    Plasmid
    #169142
    Purpose
    Constitutive expression of EGFP and sgRNAs targeted to EGFP for measurement of activity of AsCas12a\EnAsCas12a.
    Depositor
    John Doench, David Root
    Insert
    EGFP , EGFP sgRNA
    Use
    CRISPR and Lentiviral
    Available since
    June 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    169142-plasmid-map-sequence-id-334957

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  9. PB-CAG-mRuby3-Gal8-P2A-Zeo

    Plasmid
    #150815
    Purpose
    Mammalian expression of mRuby3-Galectin8 (Gal8) fusion protein
    Depositor
    Jordan Green
    Publication
    Rui et al Sci Adv. 2022 Jan 7;8(1):eabk2855. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mRuby3-Gal8-P2A-Zeo (LGALS8 Synthetic, Human)
    Tags
    Gal8 is fused to the c-terminus of mRuby3
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    150815-plasmid-map-sequence-id-307799

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  11. pCW57.1 Lysosomal-METRIQ

    Plasmid
    #135401
    Purpose
    Expresses lysosomal-METRIQ (DNAse II-sfGFP together with cytosolic RFP) to measure lysosomal activity
    Depositor
    Eisuke Itakura
    Publication
    Ishii et al Sci Rep. 2019 Aug 12;9(1):11635. doi: 10
    Insert
    DNAse II alpha (DNASE2 Human)
    Use
    Lentiviral
    Tags
    T2A, mCherry, and sfGFP
    Expression
    Mammalian
    Mutation
    Single-nucleotide polymorphism C432T
    Available since
    Feb. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    135401-plasmid-map-sequence-id-273129

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  16. pDONR221_SLC7A5

    Plasmid
    #132197
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Publication
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SLC7A5 (SLC7A5 Human)
    Expression
    Mammalian
    Available since
    Oct. 28, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    132197-plasmid-map-sequence-id-261168

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  19. pSL0284 (pQCascade_entry)

    Plasmid
    #130635
    Purpose
    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array contains two BsaI sites for spacer cloning.
    Depositor
    Samuel H. Sternberg
    Publication
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    130635-plasmid-map-sequence-id-255022

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Showing: 9181 - 9200 of 179844 results