pCW57.1 Lysosomal-METRIQ
(Plasmid #135401)

• Purpose: Expresses lysosomal-METRIQ (DNAse II-sfGFP together with cytosolic RFP) to measure lysosomal activity
• Depositing Lab: Eisuke Itakura
• Publication: Ishii et al Sci Rep. 2019 Aug 12;9(1):11635. doi: 10.1038/s41598-019-48131-2.

Backbone

• Vector backbone: pMRX-IP
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: DNAse II alpha
• Alt name: DNASE2
• Alt name: Deoxyribonuclease-2-alpha
• Species: H. sapiens (human)
• Insert Size (bp): 1080
• Mutation: Single-nucleotide polymorphism C432T
• GenBank ID: NM_001375.2
• Entrez Gene: DNASE2 (a.k.a. DNASE2A, DNL, DNL2)
• Tags / Fusion Proteins:
  • sfGFP (C terminal on insert)
  • T2A (C terminal on insert)
  • mCherry

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GATCGCCTGGAGAATTGGaccATGggcatcccgctgctgctggcag

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry