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pSMALB-ATF4.5
(Plasmid #155032)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 155032 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSMALB
  • Backbone manufacturer
    Peter van Galen
  • Total vector size (bp) 9456
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    ATF4 5' UTR
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    277
  • Entrez Gene
    Atf4 (a.k.a. Atf-4, C/ATF, CREB-2, CREB2, TAXREB67)

Gene/Insert 2

  • Gene/Insert name
    ATF4-eGFP fusion ORF
  • Insert Size (bp)
    858

Gene/Insert 3

  • Gene/Insert name
    TagBFP
  • Insert Size (bp)
    702

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    5' UTR and ATF4-GFP fusion was clones from Addgene Plasmid #21852, "ATF4 5: 5’ATF4.GFP".

Terms and Licenses

Depositor Comments

Also used in van Galen et al., 2018, Integrated Stress Response Activity Marks Stem Cells in Normal Hematopoiesis and Leukemia, Cell Reports 25, 1109–1117 (https://doi.org/10.1016/j.celrep.2018.10.021).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSMALB-ATF4.5 was a gift from John Dick & Peter van Galen (Addgene plasmid # 155032 ; http://n2t.net/addgene:155032 ; RRID:Addgene_155032)
  • For your References section:

    The unfolded protein response governs integrity of the haematopoietic stem-cell pool during stress. van Galen P, Kreso A, Mbong N, Kent DG, Fitzmaurice T, Chambers JE, Xie S, Laurenti E, Hermans K, Eppert K, Marciniak SJ, Goodall JC, Green AR, Wouters BG, Wienholds E, Dick JE. Nature. 2014 Jun 12;510(7504):268-72. doi: 10.1038/nature13228. Epub 2014 Apr 28. 10.1038/nature13228 PubMed 24776803