164,509 results

1. qTAG-N-Solo-mStayGold-CEP135

   Plasmid

   #227287

   Purpose

   Donor template for mStayGold insertion into the N-terminus of the CEP135 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px330-CEP135 (Addgene #227286)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   CEP135 Homology Arms flanking a mStayGold Tag (CEP135 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-PITCh-MAPRE1

   Plasmid

   #207793

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of MAPRE1 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of MAPRE1 (MAPRE1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330-CEP135

   Plasmid

   #227286

   Purpose

   Expresses SpCas9 and a sgRNA targeting the N-terminus of CEP135 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of CEP135 (CEP135 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-cT3G-cERP2-PAX6a

   Plasmid

   #222576

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of PAX6a

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   PAX6a (PAX6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF3505

   Plasmid

   #144981

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   KLF12 (KLF12 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV hSYN DIO mGluR1-myc-FLAG

   Plasmid

   #166227

   Purpose

   AAV expressing cre-dependent mGluR1-myc-FLAG

   Depositor

   Jason Aoto

   Article

   Cai et al Neuron. 2021 Apr 7;109(7):1137-1149.e5.

   Insert

   metabotropic glutamate receptor 1 (Grm1 Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Myc and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   human Synapsin

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJRH-1364 LV EF1a-CD4 IRES-EGFP

   Plasmid

   #201920

   Purpose

   2nd generation lentiviral transfer plasmid for engineering cells to express human CD4

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Insert

   CD4 (CD4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. H3K9me3 biosensor

   Plasmid

   #120802

   Purpose

   FRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRET

   Depositor

   Yingxiao Wang

   Article

   Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

   Insert

   Truncated YPet-HP1-EV linker-ECFP-mouse histone H3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCFT4.2

   Plasmid

   #208807

   Purpose

   Expresses the fluorescent protein phiLOV2.1

   Depositor

   François-Xavier Campbell-Valois

   Article

   Tchagang et al Front Microbiol. 2023 Oct 20;14:1245755.

   Insert

   phiLOV2.1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. C1QBP-bio-His

    Plasmid

    #53330

    Purpose

    Expresses full-length Complement component 1 Q subcomponent-binding protein ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    C1QBP (C1QBP Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG_eGCaMP(2+)

    Plasmid

    #201148

    Purpose

    expresses eGCaMP2+ in mammalian cells

    Depositor

    Andre Berndt

    Article

    Wait et al Res Sq. 2023 Aug 7:rs.3.rs-3146778. doi:

    Insert

    eGCaMP2+
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXs GFP-LC3-RFP

    Plasmid

    #117413

    Purpose

    Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux without any drug resistance.

    Depositor

    Noboru Mizushima

    Article

    Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    EGFP and mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. NLS-mCherry-LEXY (pDN122)

    Plasmid

    #72655

    Purpose

    Blue light-inducible nuclear export of mCherry

    Depositor

    Roland Eils, Barbara Di Ventura

    Article

    Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

    Insert

    NLS-mCherry-LEXY
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. IST1-GFP

    Plasmid

    #186298

    Purpose

    Expresses human IST1 C-terminally fused to GFP

    Depositor

    Geert van den Bogaart

    Article

    Stempels et al J Cell Biol. 2023 Jul 3;222(7):e20220513

    Insert

    IST1 homolog (IST1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    GFP

    Promoter

    CMV

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CAG-H2B tdTomato

    Plasmid

    #116870

    Purpose

    Nuclear localized tdTomato

    Depositor

    Loren Looger

    Article

    Economo et al Nature. 2018 Nov;563(7729):79-84. doi: 1

    Insert

    H2B tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC1-CMV-mito-DAAO

    Plasmid

    #141132

    Purpose

    Expresses DAAO with mitochondrial localization signal in mammalian cells

    Depositor

    Vsevolod Belousov

    Insert

    mito-DAAO
    

    Use

    Tags

    2 mitochondrial localization signals and 7xHis

    Expression

    Mammalian

    Mutation

    deleted DAAO amino acids 367-368

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-myc-GLUT4-mCherry

    Plasmid

    #64049

    Purpose

    3rd generation lentiviral transfer plasmid. Reporter for GLUT4 translocation

    Depositor

    Weiping Han

    Article

    Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

    Insert

    GLUT4 (Slc2a4 Mouse)
    

    Use

    Lentiviral

    Tags

    c-Myc and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available Since

    July 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRISPathBrick

    Plasmid

    #65006

    Purpose

    E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks

    Depositor

    Mattheos Koffas

    Article

    Cress et al ACS Synth Biol. 2015 Mar 30.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive native promoters

    Available Since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. HA-MBP-TEVpCS-ADGRA3 CTFdeltaTA-FLAG

    Plasmid

    #217713

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRA3 (ADGRA3 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-743

    Promoter

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. HA-MBP-TEVpCS-ADGRA2 CTFdeltaTA-FLAG

    Plasmid

    #217712

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRA2 (ADGRA2 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-752

    Promoter

    Available Since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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