166,276 results

1. shp53 pLKO.1 puro

   Plasmid

   #19119

   Purpose

   3rd gen lentiviral vector for knocking down p53 gene expression

   Depositor

   Bob Weinberg

   Article

   Godar et al Cell. 2008 Jul 11. 134(1):62-73.

   Insert

   small hairpin RNA against tumor protein p53 (TP53 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Sept. 9, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSPTg.T2FpAXK (#52)

   Plasmid

   #35962

   Depositor

   Thomas Sato

   Article

   Schlaeger et al Proc Natl Acad Sci U S A. 1997 Apr 1;94(

   Inserts

   Tie2 promoter
   SV40 pA
   Tie2 enhancer
   

   Available Since

   July 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-EF1A-Puro-hTBK1-E696K-EGFP

   Plasmid

   #221554

   Purpose

   Expresses GFP-tagged human TBK1 with E696K mutation in mammalian cells. Compatible with piggybac transposase for genome integration.

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Piggybac

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Changed glutamate 696 to lysine

   Promoter

   EF1a

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pgRNA-CKB

   Plasmid

   #73501

   Purpose

   gRNA expression vector (with mKate2)

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Insert

   mKate2-T2A-Bsd
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. TwinStrepSUMO-pvc10

   Plasmid

   #243750

   Purpose

   For affinity purification of spike tip (Pvc10)

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

   Insert

   pvc10
   

   Expression

   Bacterial

   Available Since

   Sept. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDRM98 mKate2 Blast

   Plasmid

   #174720

   Purpose

   Expresses the NLS-tagged mKate2 gene, P2A and the blasticidin resistance gene.

   Depositor

   Wayne Tilley

   Article

   Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

   Inserts

   mKate2
   BSD
   

   Use

   Lentiviral

   Tags

   P2A linker and SV40 NLS with GGS linker

   Expression

   Mammalian

   Promoter

   MND and MND (off mKate-P2A)

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLumi-LgBiT

   Plasmid

   #188319

   Purpose

   Expression of LgBiT via sigma 70 promoter in E. coli

   Depositor

   Kate Adamala

   Article

   Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

   Insert

   LgBiT
   

   Use

   Luciferase and Synthetic Biology

   Expression

   Bacterial

   Promoter

   sigma 70 promoter

   Available Since

   Sept. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. p414-TEF1p-Cas9-CYC1t

   Plasmid

   #43802

   Purpose

   A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoter

   Depositor

   George Church

   Article

   Dicarlo et al Nucleic Acids Res

   Insert

   Human Optimized S. pyogenes Cas9
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   TEF1 promoter

   Available Since

   March 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-ICUE4

   Plasmid

   #181846

   Purpose

   Fourth-generation FRET-based Indicator of cAMP Using Epac. Genetically encoded cAMP indicator. Contains high-sensitivity Epac Q270E mutation.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   ICUE4 (RAPGEF3 Human)
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Glutamine 122 in Epac1 domain mutated to glutamat…

   Promoter

   CMV

   Available Since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMasterK

    Plasmid

    #165081

    Purpose

    Episomal expression of reprogramming factors.

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Stem Cell Res. 2021 May;53:102380. doi:

    Inserts

    OCT4 (POU5F1 Human)
    SOX2 (SOX2 Human)
    KLF4 (KLF4 Human)
    MYC (MYC Human)
    LRH1 (NR5A2 Human)
    NANOG (NANOG Human)
    lin28 (LIN28A Human)
    EGFP
    HSV TK
    KDM4D (KDM4D Callithrix jacchus)
    GLIS1 (GLIS1 Human)
    

    Use

    Episomal

    Expression

    Mammalian

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. sfTq2ox-PBP5

    Plasmid

    #117960

    Purpose

    Periplasmic expression of superfolder mTurquoise2ox (pNM077 pTHV037-DsbAss-LEGPAGL-sfTq2ox-EFGS-∆1-17-PBP5)

    Depositor

    Tanneke den Blaauwen

    Article

    Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

    Insert

    sfTq2ox-PBP5 (dacA )
    

    Expression

    Bacterial

    Available Since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSLIK-IDH1-R132H-FLAG

    Plasmid

    #66803

    Purpose

    Tet-inducible expression of mutant IDH1 in mammalian cells, 3rd generation lentiviral vector

    Depositor

    Christian Metallo

    Article

    Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

    Insert

    IDH1 (IDH1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R132H

    Promoter

    CMV

    Available Since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. KRAB-dCas9

    Plasmid

    #112195

    Purpose

    KRAB fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Insert

    KRAB (ZNF10 Human)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-KRAB-dCas9-NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAID-EF1a linearizing in pX330

    Plasmid

    #140610

    Purpose

    Crispr/Cas9 plasmid for linearization of pAID-EF1a

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    sgRNA for pAID-EF1a plasmid digestion
    

    Use

    CRISPR

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pInducer10-mir-RUP-PheS

    Plasmid

    #44011

    Purpose

    Lentiviral shRNA vector (pGIPz based) for inducible knockdown

    Depositor

    Stephen Elledge

    Article

    Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

    Insert

    PheS Gly294
    

    Use

    Lentiviral

    Tags

    RFP

    Expression

    Mammalian

    Promoter

    TRE2

    Available Since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. hACE2

    Plasmid

    #1786

    Purpose

    Mammalian expression of human ACE2

    Depositor

    Hyeryun Choe

    Article

    Li et al Nature. 2003 Nov 27. 426(6965):450-4.

    Has Service

    Cloning Grade DNA

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    C9 (GTETSQVAPA)

    Expression

    Mammalian

    Available Since

    Feb. 14, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pDONR223_PAK1_WT

    Plasmid

    #82220

    Purpose

    Gateway Donor vector containing PAK1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PAK1 (PAK1 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. B52 (empty plasmid backbone to express 2 sgRNAs)

    Plasmid

    #100708

    Purpose

    Empty plasmid backbone to express 2 sgRNAs (use BbsI and BsmBI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoters

    Available Since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOpen-NotIR (GC)

    Plasmid

    #165507

    Purpose

    Type II restriction enzyme that recognizes the double-stranded sequence GCGGCCGC and cleaves after C-1.

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    NotI (GC-rich recognition site)
    

    Use

    Synthetic Biology

    Available Since

    July 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pCMV-GST-IRP1

    Plasmid

    #216827

    Purpose

    Expresses human IRP1 with a N-terminal GST-tag in mammalian cells.

    Depositor

    Samie Jaffrey

    Insert

    ACO1 (ACO1 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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