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pminiTol2

Plasmid

#31829

Purpose

Vector with minimal Tol2 transposition sites, with capacity for delivering larger gene cargo than standard Tol2 vectors

Depositor

Stephen Ekker

Article

Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

Type

Empty backbone

Use

Cloning vector

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-GFPg1 (BB09)

Plasmid

#139461

Purpose

Lentiviral vector with gRNA targeting GFP; includes puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

GFP-targeting sgRNA inserted; resistance gene: puroR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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SP-Cas9

Plasmid

#62731

Purpose

Expresses SP-Cas9 protein in bacterial cells

Depositor

Niels Geijsen

Article

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

SP-CAS9

Use

CRISPR

Tags

His tag and NLS

Expression

Bacterial

Mutation

Codon optimazed for e.coli

Promoter

T7

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_CalR_HaloTag7_KDEL

Plasmid

#175530

Purpose

Expression of HaloTag7 in the endoplasmic reticulum of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

CalR-HaloTag7-KDEL

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pRPA32-mCherry

Plasmid

#211384

Purpose

mammalian expression of human RPA32 C-terminally fused to mCherry

Depositor

Helmut Pospiech

Insert

RPA32 (RPA2 Human)

Tags

mCherry

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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LAMP1-mRFP-FLAG

Plasmid

#34611

Purpose

3rd gen lentiviral vector expressing a fusion protein of Lamp1, mRFP, and a FLAG tag

Depositor

David Sabatini

Article

Zoncu et al Science. 2011 Nov 4;334(6056):678-83.

Insert

LAMP1 (Lamp1 Rat)

Use

Lentiviral

Tags

2xFlag and mRFP

Expression

Mammalian

Available Since

March 20, 2012

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-EGFP-NLS-FLAG- cGAS E225A/D227A

Plasmid

#127660

Purpose

Over-express catalytically inactive EGFP-NLS-FLAG-cGAS E225A/D227A

Depositor

Nicolas Manel

Article

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

cGAS (CGAS Human)

Use

Lentiviral

Mutation

E225A/D227A

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pUC18R6K-mini-Tn7T-Gm

Plasmid

#65022

Purpose

GmR mini-Tn7 vector, R6K replicon

Depositor

Herbert Schweizer

Article

Choi et al Nat Protoc. 2006;1(1):153-61.

Type

Empty backbone

Available Since

Aug. 17, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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GFP-Rab27A

Plasmid

#89237

Purpose

Expression of GFP-tagged human Rab27A in mammalian cells

Depositor

William Gahl

Article

Westbroek et al Mol Genet Metab. 2008 Jun;94(2):248-54.

Insert

human Rab27A (RAB7A Human)

Tags

eGFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-cdk5GFP

Plasmid

#1346

Depositor

Li-Huei Tsai

Insert

CDK5 (CDK5 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

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pET-Sac-Abeta(M1-42)

Plasmid

#71875

Purpose

Expresses the amyloid-beta protein (42 aa), containing an exogenous methionine (Abeta(M1-42)), when expressed in BL21* DE3 p LysS E. coli cells

Depositor

Dominic Walsh

Article

Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

Insert

Abeta(M1-42) (APP Human)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP-IpgD

Plasmid

#183666

Purpose

N-terminally tagged Shigella flexneri IpgD for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

IpgD

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_YWHAB_14-3-3

Plasmid

#109904

Purpose

Protein expression and purification of YWHAB_14-3-3

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

YWHAB_14-3-3 (YWHAB Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG-Ku80

Plasmid

#46958

Purpose

Plasmid for expression of GFP-FLAG tagged human Ku80 (N-terminal tag). Confers resistance to G418.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Insert

Ku80 (XRCC5 Human)

Tags

GFP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pCBC-DT3T4

Plasmid

#50592

Purpose

CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Insert

T3, gRNA scaffold, U6-1tplus, U6-26p, T4

Use

CRISPR; Pcr template

Expression

Plant

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pET His6 Thioredoxin TEV LIC cloning vector (2T-T)

Plasmid

#29712

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6-Thioredoxin-TEV

Expression

Bacterial

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pHTN_HaloTag_KIF5B[1-560]

Plasmid

#213403

Purpose

Truncated human kinesin-1 with HaloTag at the N-terminus

Depositor

Jonas Ries

Article

Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

Insert

HaloTag-KIF5B[1-560] (KIF5B Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_p.G12D

Plasmid

#81651

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway Cloning

Mutation

G12D

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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CAG-GFP/cre

Plasmid

#49054

Purpose

Retroviral vector contains CAG promoter , CMV enhancer and a large synthetic intron that used to allow the ubiquitous expression of GFP-fused Cre recombinase

Depositor

Fred Gage

Article

Tashiro et al Nat Protoc. 2006;1(6):3049-55.

Insert

GFP/Cre

Use

Retroviral

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-HA-rM3D(Gs)-IRES-mCitrine

Plasmid

#50468

Purpose

Gs-coupled rM3D-IRES-mCitrine under the control of CaMKIIa promoter

Depositor

Bryan Roth

Insert

rM3D-IRES-mCitrine

Use

AAV

Tags

HA

Mutation

See supplemental documents for DREADD mutations

Promoter

CaMKIIa

Available Since

April 16, 2014

Availability

Academic Institutions and Nonprofits only

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