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pDD315 (mTurquoise2^SEC^2xHA)
(Plasmid #73343)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73343 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
    pUC19 (modified)
  • Backbone size w/o insert (bp) 2600
  • Total vector size (bp) 10500
  • Modifications to backbone
    Addition of ccdB markers to facilitate homology arm cloning
  • Vector type
    Worm Expression, Cre/Lox, CRISPR
  • Selectable markers
    Hygromycin ; sqt-1(d) (worm phenotypic marker)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Turbo
  • Growth instructions
    The dual ccdB sites in this vector may make it prone to recombination. It is recommended to pick several single clones and test by restriction digestion before use. This construct should be maintained as a purified plasmid stock in addition to a bacterial stock in case there is a need to re-transform.
  • Copy number


  • Gene/Insert name
  • Species
    C. elegans (nematode), Synthetic
  • Insert Size (bp)
  • Tags / Fusion Proteins
    • C. elegans codon-optimized mTurquoise2
    • 2xHA

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer M13 Forward (tgtaaaacgacggccagt)
  • 3′ sequencing primer M13 Reverse (caggaaacagctatgaccatg)
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pDD315 is a derivative of our published vectors pDD282-285 (Dickinson et al. Genetics 2015). It has a 2xHA tag in place of 3xFlag, and Lox511I sites in place of LoxP. These features allow pDD315 to be used in a genetic background that has already been modified using a green or red FP-SEC vector, without conflicts between epitope tags and Lox sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDD315 (mTurquoise2^SEC^2xHA) was a gift from Bob Goldstein (Addgene plasmid # 73343 ; ; RRID:Addgene_73343)
  • For your References section:

    Architecture of the cortical actomyosin network driving apical constriction in C. elegans. Zhang P, Medwig-Kinney TN, Goldstein B. J Cell Biol. 2023 Sep 4;222(9):e202302102. doi: 10.1083/jcb.202302102. Epub 2023 Jun 23. 10.1083/jcb.202302102 PubMed 37351566