PurposeTo see the activity of HBV transcription using Luciferase assay
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||71414||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3100
- Total vector size (bp) 5600
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Gene/Insert nameHBV core promoter, enhancer I, enhancer II and luciferase
Insert Size (bp)2500
- Promoter HBV
- Cloning method Unknown
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHBV-Luc was a gift from Wang-Shick Ryu (Addgene plasmid # 71414 ; http://n2t.net/addgene:71414 ; RRID:Addgene_71414)
For your References section:Stimulation of hepatitis B virus genome replication by HBx is linked to both nuclear and cytoplasmic HBx expression. Cha MY, Ryu DK, Jung HS, Chang HE, Ryu WS. J Gen Virol. 2009 Apr;90(Pt 4):978-86. doi: 10.1099/vir.0.009928-0. Epub 2009 Mar 4. 10.1099/vir.0.009928-0 PubMed 19264639