pMB1610_pRR-Puro
(Plasmid #65853)

• Purpose: Puromycin-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9
• Depositing Lab: Marc Bühler
• Publication: Flemr et al Cell Rep. 2015 Jul 28;12(4):709-16. doi: 10.1016/j.celrep.2015.06.051. Epub 2015 Jul 16.

Backbone

• Vector backbone: phRL-SV40
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3705
• Total vector size (bp): 3583
• Modifications to backbone: Renilla Luciferase coding sequence replaced with split puromycin N-acetyltransferase
• Vector type: Mammalian Expression, CRISPR, TALEN

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: split puromycin N-acetyltransferase coding sequence
• Alt name: split PuroR
• Promoter: SV40

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: AatII (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry