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A split horseradish peroxidase for the detection of intercellular protein-protein interactions and sensitive visualization of synapses.
Martell JD, Yamagata M, Deerinck TJ, Phan S, Kwa CG, Ellisman MH, Sanes JR, Ting AY
Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
73143CAG sHRPa-NRXLarge fragment of split HRP fused to the extracellular terminus of neurexin3B. CAG promoter.
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73144CAG sHRPb-NLGSmall fragment of split HRP fused to the extracellular terminus of NLG1. CAG promoter.
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73145CAG sHRPa-FRB-pre mGRASPLarge fragment of split HRP and FRB fused to the extracellular terminus of the "pre mGRASP" scaffold published by Magee, in which the extracellular domain of NRX is replaced by portions of CD4.
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73146CAG sHRPb-FKBP-post mGRASPSmall fragment of split HRP and FKBP fused to the extracellular terminus of the "post mGRASP" scaffold published by Magee, in which the majority of the NLG1 extracellular domain is deleted.
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73147Synapsin sHRPa-NRX in FSW lentiviral vectorLarge fragment of split HRP fused to the extracellular terminus of neurexin3B. This is a lentiviral plasmid, but can also be used for transfections. Syanpsin promoter.
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73148Synapsin sHRPb-NLG in FSW lentiviral vectorSmall fragment of split HRP fused to the extracellular terminus of NLG1. This is a lentiviral plasmid, but can also be used for transfections. Syanpsin promoter.
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73149pDisplay sHRPa-KDELLarge fragment of split HRP targeted for expression in the lumen of the endoplasmic reticulum (ER). CMV promoter.
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73150pDisplay sHRPb-KDELSmall fragment of split HRP targeted for expression in the lumen of the endoplasmic reticulum (ER). CMV promoter.
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73151YIP sHRPa-Aga1PLarge fragment of split HRP fused to the N-terminus of the yeast mating protein Aga1P. When linearized, this vector incorporates into the yeast genome.
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73152pCTCON2 Aga2P-HRPFull-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.
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79910pXL-CAG-ICeuI-sHRPaNRX3betapiggyBac transposon vector, sHRPaNRX3beta
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79912pXL-CAG-ICeuI-Cerulean-2A-sHRPbNLG1piggyBac transposon vector, Cerulean-2A-sHRPbNLG1
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79913pXL-BacII-CAG-Zeo-t2A-sHRPaNRX3betapiggyBac transposon vector, Zeocin-2A-sHRPaNRX3beta
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79914pXL-BacII-CAG-Zeo-t2A-sHRPaNLG1piggyBac transposon vector, Zeocin-2A-sHRPbNLG1
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Recombinant Antibodies from Article