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Synapsin sHRPb-NLG in FSW lentiviral vector
(Plasmid #73148)


Item Catalog # Description Quantity Price (USD)
Plasmid 73148 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    FSW lentiviral vector
  • Backbone size w/o insert (bp) 8451
  • Total vector size (bp) 11337
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number


  • Gene/Insert name
  • Species
    M. musculus (mouse), Synthetic
  • Insert Size (bp)
  • Entrez Gene
    Nlgn1 (a.k.a. 6330415N05Rik, NL1, Nlg1, mKIAA1070)
  • Promoter Synapsin
  • Tag / Fusion Protein
    • V5 tag

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer CTCCCCTTCCCGGCCACCTTGGTCG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

sHRPb is the small split HRP fragment. It consists of amino acids 214-308 of horseradish peroxidase (HRP) with the following 2 mutations: N255D, L299R.

The plasmid contains the following features (including promoter region):
PacI-SynapsinP-XbaI-NLG1 ss-AgeI-sHRPb-5 aa linker-V5 epitope tag-MfeI-NLG1-stop-AscI

Human synapsin promoter
5 aa linker: GSGSG
V5 epitope tag: GKPIPNPLLGLDST
Lentiviral vector. Derived from FCGW with human synapsin1 promoter replacing CMV

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Synapsin sHRPb-NLG in FSW lentiviral vector was a gift from Alice Ting (Addgene plasmid # 73148 ; ; RRID:Addgene_73148)
  • For your References section:

    A split horseradish peroxidase for the detection of intercellular protein-protein interactions and sensitive visualization of synapses. Martell JD, Yamagata M, Deerinck TJ, Phan S, Kwa CG, Ellisman MH, Sanes JR, Ting AY. Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563. 10.1038/nbt.3563 PubMed 27240195