Synapsin sHRPb-NLG in FSW lentiviral vector
PurposeSmall fragment of split HRP fused to the extracellular terminus of NLG1. This is a lentiviral plasmid, but can also be used for transfections. Syanpsin promoter.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||73148||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneFSW lentiviral vector
- Backbone size w/o insert (bp) 8451
- Total vector size (bp) 11337
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
SpeciesM. musculus (mouse), Synthetic
Insert Size (bp)2886
Entrez GeneNlgn1 (a.k.a. 6330415N05Rik, NL1, Nlg1, mKIAA1070)
- Promoter Synapsin
/ Fusion Protein
- V5 tag
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer CTCCCCTTCCCGGCCACCTTGGTCG (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
sHRPb is the small split HRP fragment. It consists of amino acids 214-308 of horseradish peroxidase (HRP) with the following 2 mutations: N255D, L299R.
The plasmid contains the following features (including promoter region):
PacI-SynapsinP-XbaI-NLG1 ss-AgeI-sHRPb-5 aa linker-V5 epitope tag-MfeI-NLG1-stop-AscI
Human synapsin promoter
5 aa linker: GSGSG
V5 epitope tag: GKPIPNPLLGLDST
Lentiviral vector. Derived from FCGW with human synapsin1 promoter replacing CMV
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Synapsin sHRPb-NLG in FSW lentiviral vector was a gift from Alice Ting (Addgene plasmid # 73148 ; http://n2t.net/addgene:73148 ; RRID:Addgene_73148)
For your References section:A split horseradish peroxidase for the detection of intercellular protein-protein interactions and sensitive visualization of synapses. Martell JD, Yamagata M, Deerinck TJ, Phan S, Kwa CG, Ellisman MH, Sanes JR, Ting AY. Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563. 10.1038/nbt.3563 PubMed 27240195