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pET28-Dnmt3a3L-sc27
(Plasmid #71827)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71827 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Dnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion
  • Alt name
    Mouse Dnmt3a catalytic domain
  • Alt name
    Mouse Dnmt3L C-terminal domain
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1700
  • Entrez Gene
    Dnmt3a (a.k.a. MmuIIIA)
  • Tag / Fusion Protein
    • 6xHis tag (N terminal on backbone)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gene origin for Dnmt3a full length was Dr. En Li (Cardiovascular Research Center, Massachusetts General Hospital, Department of Medicine, Harvard Medical School, Charlestown, MA 02129, USA).
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

For protein production, use BL21 Rosetta (DE3) cells.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-Dnmt3a3L-sc27 was a gift from Albert Jeltsch (Addgene plasmid # 71827 ; http://n2t.net/addgene:71827 ; RRID:Addgene_71827)
  • For your References section:

    Targeted methylation and gene silencing of VEGF-A in human cells by using a designed Dnmt3a-Dnmt3L single-chain fusion protein with increased DNA methylation activity. Siddique AN, Nunna S, Rajavelu A, Zhang Y, Jurkowska RZ, Reinhardt R, Rots MG, Ragozin S, Jurkowski TP, Jeltsch A. J Mol Biol. 2013 Feb 8;425(3):479-91. doi: 10.1016/j.jmb.2012.11.038. Epub 2012 Dec 4. 10.1016/j.jmb.2012.11.038 PubMed 23220192