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pCS2-epNTR
(Plasmid #62213)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 62213 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCS2
  • Backbone size w/o insert (bp) 4100
  • Total vector size (bp) 4725
  • Vector type
    mRNA synthesis

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    epNTR
  • Alt name
    zebrafish optimized nfsB.zf1 with T41L N71S F124W
  • Species
    Synthetic
  • Insert Size (bp)
    654

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (dam) (not destroyed)
  • 5′ sequencing primer sp6
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-epNTR was a gift from Harold Burgess (Addgene plasmid # 62213 ; http://n2t.net/addgene:62213 ; RRID:Addgene_62213)
  • For your References section:

    Direct activation of the Mauthner cell by electric field pulses drives ultrarapid escape responses. Tabor KM, Bergeron SA, Horstick EJ, Jordan DC, Aho V, Porkka-Heiskanen T, Haspel G, Burgess HA. J Neurophysiol. 2014 Aug 15;112(4):834-44. doi: 10.1152/jn.00228.2014. Epub 2014 May 21. 10.1152/jn.00228.2014 PubMed 24848468