166,276 results

1. p(+)MV-NSE-FlagP-M502-3p

   Plasmid

   #58748

   Purpose

   Expresses recombinant measles virus with Flag-P gene tagged si2 (siP) target sequence

   Depositor

   Branka Horvat

   Insert

   Measles virus antigenome
   

   Tags

   Flag

   Promoter

   T7

   Available Since

   Aug. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. mNeonGreen-APEX3

   Plasmid

   #202590

   Purpose

   Gamma-retroviral vector that expresses mNeonGreen fused to APEX3.

   Depositor

   Reuben Harris

   Article

   Becker et al J Mol Biol. 2023 May 12:168145. doi: 10.

   Insert

   mNeonGreen-APEX3-V5 (APX1 )
   

   Use

   Retroviral

   Tags

   V5 epitope and mNeonGreen

   Expression

   Mammalian

   Mutation

   APEX2 L242A

   Promoter

   MESV LTR

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Raptor_1 shRNA

   Plasmid

   #1857

   Depositor

   David Sabatini

   Article

   Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

   Insert

   Raptor (RPTOR Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   April 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRX-INU-TEX264-FLAG

   Plasmid

   #128258

   Purpose

   Expresses TEX264 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

   Insert

   TEX264 (TEX264 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cas9 TLS1

   Plasmid

   #196977

   Purpose

   Expresses Cas9 fused with TLS1 mobility signal that confers graft mobility. Induced by estradiol.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   Cas9 with TLS1 tag
   

   Expression

   Plant

   Promoter

   pG10-90

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. CoV2-Spike-D614G

   Plasmid

   #177960

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
   

   Expression

   Mammalian

   Mutation

   D614G

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. HSP70-pp7i-mCh-UPG

   Plasmid

   #161011

   Purpose

   C-terminal mCherry fusion to Arabidopsis HSP70 with intronic PP7

   Depositor

   Krishna Niyogi

   Article

   Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

   Insert

   PP7 and H2B-mScarlet
   

   Expression

   Plant

   Available Since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV5-Flag-RhoA wt

   Plasmid

   #11750

   Depositor

   Jeff Wrana

   Article

   Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

   Insert

   RhoA (RHOA Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Aug. 4, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pD3sE_SC12

   Plasmid

   #219720

   Purpose

   DV3 E dimer with fusion loop mutation and increased expression

   Depositor

   Brian Kuhlman

   Article

   Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi

   Insert

   DV3 sE SC12
   

   Tags

   8xHis and human serum albumin (HSA)

   Expression

   Mammalian

   Mutation

   G106D, A257W, T260R, F277W, A278P, G29K, T33V, A3…

   Available Since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-BRX-eGFP-NLS

    Plasmid

    #217534

    Purpose

    AAV vector to drive the expression of eGFP under the control of the 4xBRE regulatory element of SMAD1 and miniXon splicing casette transcription factor in vivo

    Depositor

    Peter Scheiffele

    Article

    Okur et al bioRxiv 2023

    Insert

    BMP reporter element and miniXon casette (Smad1 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    BMP Reporter Element

    Available Since

    April 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. 0_T7-mScarlet3

    Plasmid

    #225927

    Purpose

    In vitro synthesis of mRNA for fluorescent protein mScarlet3 (control)

    Depositor

    Michalis Averof

    Article

    Karapidaki et al bioRxiv 2024.11.12.623055

    Insert

    mScarlet3
    

    Use

    In vitro transcription

    Promoter

    T7

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. BII-CMV-AfmW3A

    Plasmid

    #133402

    Purpose

    Piggybac vector for constitutive expression of Afamin + Wnt3a for production of conditioned media in HEK293T cells

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    Afamin and Wnt3a (Afm, Wnt3a Mouse)
    

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJeM1

    Plasmid

    #135088

    Purpose

    rhamnose-inducible expression vector for replication in E. coli and P. putida

    Depositor

    Josef Altenbuchner

    Article

    Jeske et al Appl Microbiol Biotechnol. 2010 Feb;85(6

    Insert

    rhaR rhaS rhaPBAD eGFP, mob (rhaR E.coli)
    

    Expression

    Bacterial

    Available Since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHN922

    Plasmid

    #178975

    Purpose

    Expression of GST-His8-GFP-nanobody

    Depositor

    Kazuhiro Abe

    Insert

    GST-His8-GFP-nanobody
    

    Use

    Affinity Reagent/ Antibody

    Tags

    GST, His8

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDZ2FE_Champ1_1-812

    Plasmid

    #105675

    Purpose

    Expresses Flag-tagged full-length human Champ1 protein

    Depositor

    Thanos Halazonetis

    Insert

    Champ1 (CHAMP1 Human)
    

    Tags

    2X Flag tag

    Expression

    Mammalian

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS_4xMma PylT_FLAG-Mma PylRS AF

    Plasmid

    #140023

    Purpose

    Plasmid with 4xMmaPylT cassette and MmaPylRS AF for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y271A, Y349F

    Promoter

    EF1

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-TRE3G-PYL1-sfGFP-PML

    Plasmid

    #167011

    Purpose

    expression of PYL1-SfGFP-PML, lentivirus vector

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    PML (PML Human)
    

    Use

    Lentiviral

    Tags

    GFP-PML

    Expression

    Mammalian

    Available Since

    June 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. iLOV-Farnesyl-5

    Plasmid

    #56516

    Purpose

    Localization: Membrane, Excitation: 450, Emission: 495

    Depositor

    Michael Davidson

    Insert

    Farnesyl (HRAS Human)
    

    Tags

    iLOV

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pAd388-shLuc

    Plasmid

    #83275

    Purpose

    shRNA targeting luciferace

    Depositor

    Joe Landry

    Article

    Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

    Insert

    shRNA to Luciferase
    

    Use

    Retroviral

    Promoter

    pUC ori

    Available Since

    Dec. 2, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. Klotho (secreted)

    Plasmid

    #17713

    Depositor

    Hal Dietz

    Article

    Arking et al Proc Natl Acad Sci U S A. 2002 Jan 22. 9

    Insert

    Klotho (KL Human)
    

    Tags

    His and V5

    Expression

    Mammalian

    Available Since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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