161,985 results

1. TRIB2 gRNA (BRDN0001144754)

   Plasmid

   #75602

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRIB2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TRIB2 (TRIB2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. TRIB2 gRNA (BRDN0001148141)

   Plasmid

   #75603

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRIB2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TRIB2 (TRIB2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-D1R

   Plasmid

   #89160

   Purpose

   Express vaccinia virus D1R in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Vaccinia virus D1R
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promotor

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-C1 EGFP-3XNLS

   Plasmid

   #58468

   Purpose

   Expresses a nuclear-targeted eGFP on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

   Insert

   3X NLS with 3X stop codons
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. plenti.CamKII.(synapto).iATPSnFR2.A95A.A119L.HaloTag

   Plasmid

   #209729

   Purpose

   Expresses presynaptic low affinity ratiometric ATP sensor

   Depositor

   Timothy Ryan

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   (synapto).iATPSnFR2.A95A.A119L.HaloTag
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CamKII

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICSL11017 (pICH47732::NOSp-BAR-NOST)

   Plasmid

   #51145

   Purpose

   Confers Basta resistance in planta

   Depositor

   Jonathan D Jones

   Insert

   NOSp-BAR-NOST
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-GABRA1-IRES2-EGFP

   Plasmid

   #170820

   Purpose

   human GABAA receptors alpha1 subunit and IRES2-EGFP

   Depositor

   Tingwei Mu

   Article

   Fu et al PLoS One. 2018 Nov 27;13(11):e0207948. d

   Insert

   Human GABAA receptor alpha1 subunit (GABRA1 Human)
   

   Use

   Tags

   IRES2-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-FLAG-CRBN-pGK-HYG

   Plasmid

   #107380

   Purpose

   Mammalian expression of FLAG-CRBN

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   cereblon (CRBN Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBA-mCherry-EGFP-PIM

   Plasmid

   #111758

   Purpose

   Low level expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 addition

   Depositor

   Lukas Kapitein

   Article

   Janssen et al Nat Commun. 2018 Oct 12;9(1):4245. doi:

   Insert

   2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Val24Glu, Tyr80Cys, Ala95Thr mutations in second …

   Promoter

   chicken beta-actin

   Available since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHaloTag-EGFP

    Plasmid

    #86629

    Purpose

    Expresses HaloTag-eGFP tandem (cytosolic)

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    HaloTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRD406

    Plasmid

    #163111

    Purpose

    Expression of mNeonGreen_H-NSdbd in eukaryotes for visualisation of nuclear DNA

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS_mNeonGreen_H-NSdbd_NLS
    

    Use

    Eukaryotic expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter; T7 promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRD399

    Plasmid

    #163106

    Purpose

    Expression of mEGFP_H-NSdbd in eukaryotes for visualisation of nuclear DNA

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS_mEGFP_H-NSdbd_NLS
    

    Use

    Eukaryotic expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter; T7 promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCXLE-Sox2-17-T2A-Klf4-E2A-Myc

    Plasmid

    #210016

    Purpose

    episomal S*KM (expressing mouse super-Sox + Klf4 + Myc) for integration-free naïve reset

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    Sox2-17-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Sox2-17 is engineered highly cooperative chimeric…

    Promoter

    CAG

    Available since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1

    Plasmid

    #138460

    Purpose

    lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRAB

    Depositor

    Jian Xu

    Article

    Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

    Insert

    MCP-KRAB-IRES-zsGreen1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. minCMV-1xmNG-dPCP

    Plasmid

    #231092

    Purpose

    Encodes a 1xmNeonGreen-dPCP under control of a weak promoter (minCMV).

    Depositor

    John Ngo

    Insert

    1xmNG-dMCP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. TOP-GFP.mC

    Plasmid

    #35491

    Depositor

    Ramesh Shivdasani

    Article

    Horst et al Cancer Res. 2012 Feb 8.

    Insert

    7xTOP-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    7xTCF/LEF

    Available since

    May 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCW-Tet-Cas9-BFP-PGK-Blast

    Plasmid

    #196720

    Purpose

    Tet-inducible expression of Cas9-T2A-TagBFP

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-T2A-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG-SV40NLS and Nucleoplasmin NLS

    Expression

    Mutation

    Promoter

    TRE, PGK

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBAD-mGreenLantern

    Plasmid

    #161913

    Purpose

    mGreenLantern fluorescent protein: bacterial expression plasmid

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    araB

    Available since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-Lyn-dPlcR

    Plasmid

    #181832

    Purpose

    Genetically encoded single-color sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells. Based on dimerization-dependent green fluorescent protein (ddGFP).

    Depositor

    Jin Zhang

    Article

    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

    Insert

    Lyn-PlcR
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase, dd…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Frt-V5-hspB8

    Plasmid

    #63109

    Purpose

    Expression of HSPB8 (small HSP) tagged with V5 in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb8 (HSPB8 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge