164,546 results

1. pPROBE-gfp[tagless]

   Plasmid

   #40164

   Depositor

   Steven Lindow

   Article

   Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

   Insert

   Promotor probe
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-hSyn-Soma-jGCaMP8m

   Plasmid

   #169257

   Purpose

   AAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8m.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Has Service

   AAV Retrograde, AAV1, and AAV9

   Insert

   Soma-jGCaMP8m
   

   Use

   AAV

   Tags

   6xHis-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19/PLIN2-GFP

   Plasmid

   #202194

   Purpose

   knock-in donor vector of GFP to the C-terminus of endogenous human PLIN2

   Depositor

   James Olzmann

   Article

   Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

   Insert

   PLIN2 (PLIN2 Human)
   

   Use

   Cloning vector

   Tags

   GFP knock-in

   Expression

   Mutation

   Promoter

   lac

   Available Since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Salsa-BC1-P1A1

   Plasmid

   #202842

   Purpose

   Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC1

   Depositor

   Noah Palm

   Article

   Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

   Insert

   EGFP/BC1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tet Response Element (TRE)

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ac5-STABLE2-puro

   Plasmid

   #208032

   Purpose

   Enables expression of 2 ORFs (with or without tags) and selection with puromycin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-puro with Actin5C promoter.

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGint

   Plasmid

   #24217

   Depositor

   Mariano Garcia-Blanco

   Article

   Bonano et al Nat Protoc. 2007 . 2(9):2166-81.

   Insert

   pGint
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Modified adenovirus in GFP

   Promoter

   Available Since

   April 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBMN-I-GFP

   Plasmid

   #1736

   Purpose

   This retroviral vector has an IRES-GFP downstream of the cloning sites

   Depositor

   Garry Nolan

   Insert

   IRES-GFP
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 20, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-CMV-Nrf2

   Plasmid

   #67636

   Purpose

   AAV vector expressing Nrf2 gene

   Depositor

   Connie Cepko

   Article

   Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

   Insert

   Nrf2 (Nfe2l2 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLJM1-Flag-Rheb

   Plasmid

   #165031

   Purpose

   expression of Flag tagged Rheb protein in mammalian cells

   Depositor

   Ken Inoki

   Article

   Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

   Insert

   Rheb (RHEB Human)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCIN BRCA2 WT

    Plasmid

    #16245

    Depositor

    Mien-Chie Hung

    Article

    Wang et al Cancer Res. 2002 Mar 1. 62(5):1311-4.

    Insert

    BRCA2 (BRCA2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBabe EGFR Del1

    Plasmid

    #32062

    Depositor

    Matthew Meyerson

    Article

    Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Del1(E746-A750)

    Promoter

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAY5 (mAID-EGFP-NLS piggyBac)

    Plasmid

    #140532

    Purpose

    mAID-EGFP-NLS piggyBac

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    mAID-EGFP-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-FLAG-HA-CAD

    Plasmid

    #46238

    Purpose

    For expression of HA and FLAG-tagged murine CAD in mammalian cells

    Depositor

    Brendan Manning

    Article

    Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

    Insert

    carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. c-myc promoter (Del 2)

    Plasmid

    #16602

    Depositor

    Bert Vogelstein

    Article

    He et al Science. 1998 Sep 4. 281(5382):1509-12.

    Insert

    c-myc promoter (Del 2) (MYC Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Del-2 (KpnI->PvuII). The KpnI site is at -1194…

    Promoter

    Available Since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pET302-6His-dCas9-Halo

    Plasmid

    #72269

    Purpose

    Encodes a Halo-labeled fusion of nuclease deficient Cas9

    Depositor

    Timothée Lionnet

    Article

    Deng et al Proc Natl Acad Sci U S A. 2015 Sep 22;11

    Insert

    His6-dCas9-Halo
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-SynI-CreOn-FlpOff-mNaChBac-P2A-EGFP

    Plasmid

    #176280

    Purpose

    Viral vector for co-expression of NaChBac and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

    Depositor

    Martin Riccomagno

    Article

    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    mNaChBac-P2A-EGFP
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin I

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid

    #196084

    Purpose

    (Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Inserts

    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Catalytically dead mutant of the Cas9 endonucleas…

    Promoter

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pXW117Ptet2-gfp

    Plasmid

    #160818

    Purpose

    aTc-responsive single-layered circuit

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

    Insert

    PJ23117-30tetR-t-Ptet2-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23117, Ptet2

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Multi-frame tag

    Plasmid

    #128610

    Purpose

    In the Multi-frame tag, HIV-1 frameshift sequence is upstream of FLAG epitopes in the 0 frame are separated from one another by SunTag epitopes in the -1 frame.

    Depositor

    Tim Stasevich

    Article

    Lyon et al Mol Cell. 2019 Jul 11;75(1):172-183.e9.

    Insert

    MF tag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-TRE-DIO-hM4Di-mCherry

    Plasmid

    #182532

    Purpose

    This plasmid is for use with neuronal cell-type selective activity tagging. The hM4Di receptor expression requires both neural activity and Cre recombinase.

    Depositor

    William Wisden

    Article

    Yu et al Science. 2022 Jul;377(6601):63-72. doi:

    Insert

    AAV transgene - hSyn promoter-tet-flex-hM4Di-cherry
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    hM4Di-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    tetO promoter

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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