|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||30195||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
This item is currently unavailable outside the US without additional regulatory approval.
A non-refundable shipping export licensing fee of $85 is required to cover Addgene’s additional processing costs.
Backbone manufacturerAddgene plasmid 12337
- Backbone size w/o insert (bp) 8000
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namehistone-GFP-2A-TVA receptor-2A-rabies glycoprotein
Alt namenuclear GFP, histone H2B-eGFP, B19G
Insert Size (bp)6400
/ Fusion Protein
- eGFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site AscI, NheI (not destroyed)
- 5′ sequencing primer LNCX
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note that this plasmid was also used in Brennand et al., Nature 2011 publication (PubMed: 21490598) under the name LV-SynP-HTG.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBOB-synP-HTB was a gift from Edward Callaway & Liqun Luo (Addgene plasmid # 30195 ; http://n2t.net/addgene:30195 ; RRID:Addgene_30195)
For your References section:Cortical representations of olfactory input by trans-synaptic tracing. Miyamichi K, Amat F, Moussavi F, Wang C, Wickersham I, Wall NR, Taniguchi H, Tasic B, Huang ZJ, He Z, Callaway EM, Horowitz MA, Luo L. Nature. 2011 Apr 14;472(7342):191-196. Epub 2010 Dec 22. 10.1038/nature09714 PubMed 21179085