pBOB-synP-HTB
(Plasmid #30195)

• Depositing Labs: Edward Callaway, Liqun Luo
• Publication: Miyamichi et al Nature. 2011 Apr 14;472(7342):191-196. Epub 2010 Dec 22.

Backbone

• Vector backbone: pCSC-SP-PW-GFP
  (Search Vector Database)
• Backbone manufacturer: Addgene plasmid 12337
• Backbone size w/o insert (bp): 8000
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: histone-GFP-2A-TVA receptor-2A-rabies glycoprotein
• Alt name: nuclear GFP, histone H2B-eGFP, B19G
• Insert Size (bp): 6400
• Tag / Fusion Protein:
  • eGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: AscI, NheI (not destroyed)
• 5′ sequencing primer: LNCX
• 3′ sequencing primer: WPRE-R

Resource Information

• Terms and Licenses:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
• Industry Terms:
  • Not Available to Industry
• Articles Citing this Plasmid:
  • 8 References

Depositor Comments

Please note that this plasmid was also used in Brennand et al., Nature 2011 publication (PubMed: 21490598) under the name LV-SynP-HTG.