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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 30195 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
Currently unavailable outside the U.S.
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCSC-SP-PW-GFP
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Backbone manufacturerAddgene plasmid 12337
- Backbone size w/o insert (bp) 8000
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Growth instructionsStbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehistone-GFP-2A-TVA receptor-2A-rabies glycoprotein
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Alt namenuclear GFP, histone H2B-eGFP, B19G
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Insert Size (bp)6400
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Tag
/ Fusion Protein
- eGFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site AscI, NheI (not destroyed)
- 5′ sequencing primer LNCX
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Resource Information
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Articles Citing this Plasmid
Depositor Comments
Please note that this plasmid was also used in Brennand et al., Nature 2011 publication (PubMed: 21490598) under the name LV-SynP-HTG.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBOB-synP-HTB was a gift from Edward Callaway & Liqun Luo (Addgene plasmid # 30195 ; http://n2t.net/addgene:30195 ; RRID:Addgene_30195) -
For your References section:
Cortical representations of olfactory input by trans-synaptic tracing. Miyamichi K, Amat F, Moussavi F, Wang C, Wickersham I, Wall NR, Taniguchi H, Tasic B, Huang ZJ, He Z, Callaway EM, Horowitz MA, Luo L. Nature. 2011 Apr 14;472(7342):191-196. Epub 2010 Dec 22. 10.1038/nature09714 PubMed 21179085