Skip to main content
Addgene

pBOB-synP-HTB
(Plasmid #30195)

Print

Ordering

This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 30195 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCSC-SP-PW-GFP
  • Backbone manufacturer
    Addgene plasmid 12337
  • Backbone size w/o insert (bp) 8000
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    histone-GFP-2A-TVA receptor-2A-rabies glycoprotein
  • Alt name
    nuclear GFP, histone H2B-eGFP, B19G
  • Insert Size (bp)
    6400
  • Tag / Fusion Protein
    • eGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site AscI, NheI (not destroyed)
  • 5′ sequencing primer LNCX
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this plasmid was also used in Brennand et al., Nature 2011 publication (PubMed: 21490598) under the name LV-SynP-HTG.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBOB-synP-HTB was a gift from Edward Callaway & Liqun Luo (Addgene plasmid # 30195 ; http://n2t.net/addgene:30195 ; RRID:Addgene_30195)

  • For your References section:

    Cortical representations of olfactory input by trans-synaptic tracing. Miyamichi K, Amat F, Moussavi F, Wang C, Wickersham I, Wall NR, Taniguchi H, Tasic B, Huang ZJ, He Z, Callaway EM, Horowitz MA, Luo L. Nature. 2011 Apr 14;472(7342):191-6. doi: 10.1038/nature09714. 10.1038/nature09714 PubMed 21179085
Related items:
Commonly requested with: