pBOB-synP-HT
(Plasmid
#30456)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 30456 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCSC-SP-PW-GFP
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Backbone manufacturerAddgene plasmid 12337
- Backbone size w/o insert (bp) 8000
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Growth instructionsStbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehistone GFP
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Alt namenuclear GFP, histone H2B-eGFP
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (unknown if destroyed)
- 3′ cloning site AscI, PmeI (unknown if destroyed)
- 5′ sequencing primer human synapsin I promoter (CCACTGGACAAGCACCCAAC)
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Articles Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBOB-synP-HT was a gift from Edward Callaway (Addgene plasmid # 30456 ; http://n2t.net/addgene:30456 ; RRID:Addgene_30456)