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Purposehuman BRN3A cDNA
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62221 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepFU Teto
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Vector typeMammalian Expression, Lentiviral
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Selectable markersZeo marker is outside the LTRs and will not be packaged into virus.
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBRN3A
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SpeciesH. sapiens (human)
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Insert Size (bp)1100
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Mutationdeleted a.a. 142-181
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GenBank ID5457
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Entrez GenePOU4F1 (a.k.a. ATITHS, BRN3A, Oct-T1, RDC-1, brn-3A)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer LNCX
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Teto BRN3A was a gift from Kristin Baldwin (Addgene plasmid # 62221 ; http://n2t.net/addgene:62221 ; RRID:Addgene_62221) -
For your References section:
Selective conversion of fibroblasts into peripheral sensory neurons. Blanchard JW, Eade KT, Szucs A, Lo Sardo V, Tsunemoto RK, Williams D, Sanna PP, Baldwin KK. Nat Neurosci. 2015 Jan;18(1):25-35. doi: 10.1038/nn.3887. Epub 2014 Nov 24. 10.1038/nn.3887 PubMed 25420069
Map uploaded by the depositor.
