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Purposemammalian expression of rat GluA1
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 64942 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCMV2
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGluA1
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SpeciesR. norvegicus (rat)
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Entrez GeneGria1 (a.k.a. GluA1, gluR-A)
- Promoter CMV
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Tag
/ Fusion Protein
- SEP (superecliptic pHluorin) (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hGH-pA-R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV2-SEP-GluA1 (M1) was a gift from Jeremy Henley (Addgene plasmid # 64942 ; http://n2t.net/addgene:64942 ; RRID:Addgene_64942) -
For your References section:
Distinct subunit-specific alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor trafficking mechanisms in cultured cortical and hippocampal neurons in response to oxygen and glucose deprivation. Blanco-Suarez E, Hanley JG. J Biol Chem. 2014 Feb 21;289(8):4644-51. doi: 10.1074/jbc.M113.533182. Epub 2014 Jan 8. 10.1074/jbc.M113.533182 PubMed 24403083
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