FAT FAK biosensor
PurposeFRET biosensor. Visualization of FAK activity at focal adhesions in live cells
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||78303||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameFAT FAK biosensor
Alt nameECFP-SH2 domain of c-Src-substrate-YPet-FAT domain from FAK
Insert Size (bp)2200
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:FAT FAK biosensor was a gift from Yingxiao Wang (Addgene plasmid # 78303 ; http://n2t.net/addgene:78303 ; RRID:Addgene_78303)
For your References section:Distinct biophysical mechanisms of focal adhesion kinase mechanoactivation by different extracellular matrix proteins. Seong J, Tajik A, Sun J, Guan JL, Humphries MJ, Craig SE, Shekaran A, Garcia AJ, Lu S, Lin MZ, Wang N, Wang Y. Proc Natl Acad Sci U S A. 2013 Nov 26;110(48):19372-7. doi: 10.1073/pnas.1307405110. Epub 2013 Nov 12. 10.1073/pnas.1307405110 PubMed 24222685
Map uploaded by the depositor.