Lyn FAK biosensor
PurposeFRET biosensor. Visualization of FAK activity at lipid rafts in live cells
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||78299||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameLyn Fak biosensor
Alt nameLipid raft targeting signal from Lyn-ECFP-SH2 domain of c-Src-substrate-YPet
Insert Size (bp)1850
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
To observe the FAK activity at DRM regions, Lyn–FAK biosensor was constructed by fusing a DRM-targeting motif containing myristoylation and palmitoylation sites (glycine and cysteine) derived from Lyn, at the N-terminus of the cytosolic FAK (Cyto-FAK) biosensor (Addgene plasmid 78300).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Lyn FAK biosensor was a gift from Yingxiao Wang (Addgene plasmid # 78299 ; http://n2t.net/addgene:78299 ; RRID:Addgene_78299)
For your References section:Detection of focal adhesion kinase activation at membrane microdomains by fluorescence resonance energy transfer. Seong J, Ouyang M, Kim T, Sun J, Wen PC, Lu S, Zhuo Y, Llewellyn NM, Schlaepfer DD, Guan JL, Chien S, Wang Y. Nat Commun. 2011 Jul 26;2:406. doi: 10.1038/ncomms1414. 10.1038/ncomms1414 PubMed 21792185
Map uploaded by the depositor.