KRas FAK biosensor
(Plasmid #78301)

• Purpose: FRET biosensor. Visualization of FAK activity at plasma membrane outside lipid rafts in live cells
• Depositing Lab: Yingxiao Wang
• Publication: Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1038/ncomms1414.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: KRas FAK biosensor
• Alt name: ECFP-SH2 domain of c-Src-substrate-YPet-CAAX Box from KRas
• Species: Synthetic
• Insert Size (bp): 1850
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A polybasic-geranylgeranyl motif (cysteine) adapted from K-Ras, was fused at the carboxy terminus of the Cyto-FAK biosensor (Addgene plasmid 78300) for the generation of KRas–FAK biosensor that can localize at the general membrane area outside DRM regions