KRas FAK biosensor
PurposeFRET biosensor. Visualization of FAK activity at plasma membrane outside lipid rafts in live cells
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||78301||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameKRas FAK biosensor
Alt nameECFP-SH2 domain of c-Src-substrate-YPet-CAAX Box from KRas
Insert Size (bp)1850
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
A polybasic-geranylgeranyl motif (cysteine) adapted from K-Ras, was fused at the carboxy terminus of the Cyto-FAK biosensor (Addgene plasmid 78300) for the generation of KRas–FAK biosensor that can localize at the general membrane area outside DRM regions
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:KRas FAK biosensor was a gift from Yingxiao Wang (Addgene plasmid # 78301 ; http://n2t.net/addgene:78301 ; RRID:Addgene_78301)
For your References section:Detection of focal adhesion kinase activation at membrane microdomains by fluorescence resonance energy transfer. Seong J, Ouyang M, Kim T, Sun J, Wen PC, Lu S, Zhuo Y, Llewellyn NM, Schlaepfer DD, Guan JL, Chien S, Wang Y. Nat Commun. 2011 Jul 26;2:406. doi: 10.1038/ncomms1414. 10.1038/ncomms1414 PubMed 21792185
Map uploaded by the depositor.