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Addgene

bZiPro
(Plasmid #86846)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 86846 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pETDUET-1
  • Backbone manufacturer
    Novagene
  • Backbone size w/o insert (bp) 5420
  • Total vector size (bp) 6107
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    NS3 1-177
  • Species
    Synthetic; Zika virus
  • Insert Size (bp)
    534
  • Mutation
    NS3 protein (residues 1–177)
  • GenBank ID
    AEN75265.1
  • Promoter T7
  • Tag / Fusion Protein
    • a hexahistidine tag and a thrombin cleavage site (N terminal on backbone)

Cloning Information for Gene/Insert 1

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer pET Upstream Primer
  • 3′ sequencing primer DuetDOWN1 Primer
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    NS2B45-96
  • Species
    Synthetic; Zika virus
  • Insert Size (bp)
    159
  • Mutation
    NS2B cofactor region (residues 45–96)
  • GenBank ID
    AEN75265.1
  • Promoter T7

Cloning Information for Gene/Insert 2

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer DuetUP2 Primer
  • 3′ sequencing primer T7R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This plasmid was jointly developed at the Lee Kong Chian School of Medicine, Nanyang Technological University, and at the Experimental Therapeutics Centre, Agency for Science, Technology and Research (A*STAR).
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    bZiPro was a gift from Dahai Luo (Addgene plasmid # 86846 ; http://n2t.net/addgene:86846 ; RRID:Addgene_86846)
  • For your References section:

    Crystal structure of unlinked NS2B-NS3 protease from Zika virus. Zhang Z, Li Y, Loh YR, Phoo WW, Hung AW, Kang C, Luo D. Science. 2016 Dec 8. pii: aai9309. 10.1126/science.aai9309 PubMed 27940580