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(Plasmid #86085)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 86085 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    I.M.A.G.E consortium (Library NIH_MGC_118, Image Id 5212903)
  • Backbone size w/o insert (bp) 5639
  • Total vector size (bp) 8305
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    human HMG-CoA Reductase isoform 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    HMGCR (a.k.a. LDLCQ3, LGMDR28, MYPLG)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BstBI (not destroyed)
  • 3′ cloning site PvuII (not destroyed)
  • 5′ sequencing primer ccggactctagcctaggccg
  • 3′ sequencing primer tcctttagaacccaatgccc
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    the I.M.A.G.E consortium (pCMV-SPORT6-hHMGCR2, Library NIH_MGC_118, Image Id 5212903),
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The pCMV-SPORT6-hHMGCR1 plasmid was obtained by inserting a fragment of human HMGCR1 in the pCMV-SPORT6-hHMGCR2 plasmid (Library NIH_MGC_118, Image Id 5212903) using BstBI and PvuII unique restriction sites.

Addgene note: This plasmid also contains a portion of the 3' UTR of HMCGR1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-SPORT6-hHMGCR1 was a gift from Anne Galy (Addgene plasmid # 86085 ; ; RRID:Addgene_86085)