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pSIN-TRE-H2BeGFP-rtTA2
(Plasmid #165494)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 165494 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRRL-cPPT-hPGKTMPrtTA-WPRE
  • Backbone manufacturer
    -
  • Backbone size w/o insert (bp) 8023
  • Total vector size (bp) 9139
  • Modifications to backbone
    -
  • Vector type
    Lentiviral
  • Selectable markers
    GFP selection by fluorescent-activated cell sorting (FACS).

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    -
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Human histone H2B
  • Alt name
    -
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1116
  • Mutation
    -
  • GenBank ID
    X00088
  • Entrez Gene
    H2BC21 (a.k.a. GL105, H2B, H2B-GL105, H2B.1, H2BE, H2BFQ, H2BGL105, H2BQ, HIST2H2BE)
  • Promoter TetO-CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba I (destroyed during cloning)
  • 3′ cloning site Nhe I (not destroyed)
  • 5′ sequencing primer TCGAGTAGGCGTGTACGGT
  • 3′ sequencing primer TGAAGAATGTGCGAGACC
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The coding sequence of histone2B fused to eGFP (H2BeGFP) was amplified from the Addgene plasmid ID: 11680 deposited by Geoff Wahl.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ABOUT BACKBONE:
The lentiviral backbone was originally designed and constructed by Barde I et al. (Barde I et al. Mol Ther. 2006. 13(2): 382-90. PMID: 16275162).

ABOUT TEST DIGESTION:
Since Xba I restriction site is lost during cloning, we recommend checking the plasmid using the enzymes: Xho I and Nhe I. You should get two fragments around 1600 bp (insert+vector) and 7500 bp (vector).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSIN-TRE-H2BeGFP-rtTA2 was a gift from Hector G. Palmer (Addgene plasmid # 165494 ; http://n2t.net/addgene:165494 ; RRID:Addgene_165494)
  • For your References section:

    TET2 controls chemoresistant slow-cycling cancer cell survival and tumor recurrence. Puig I, Tenbaum SP, Chicote I, Arques O, Martinez-Quintanilla J, Cuesta-Borras E, Ramirez L, Gonzalo P, Soto A, Aguilar S, Eguizabal C, Caratu G, Prat A, Argiles G, Landolfi S, Casanovas O, Serra V, Villanueva A, Arroyo AG, Terracciano L, Nuciforo P, Seoane J, Recio JA, Vivancos A, Dienstmann R, Tabernero J, Palmer HG. J Clin Invest. 2018 Aug 31;128(9):3887-3905. doi: 10.1172/JCI96393. Epub 2018 Aug 6. 10.1172/JCI96393 PubMed 29944140