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(Plasmid #150815)


Item Catalog # Description Quantity Price (USD)
Plasmid 150815 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 6466
  • Total vector size (bp) 7864
  • Modifications to backbone
    In-frame insertion of Gal8 to form c-terminus fusion protein with mRuby3 with P2A ribosomal skip site followed by zeocin resistance for mammalian selection
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Alt name
  • Alt name
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    LGALS8 (a.k.a. Gal-8, PCTA-1, PCTA1, Po66-CBP)
  • Promoter CAG
  • Tag / Fusion Protein
    • Gal8 is fused to the c-terminus of mRuby3 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer GCAACGTGCTGGTTATTGTG
  • 3′ sequencing primer GCAACATATGCCCATATGCTGGC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-CAG-mRuby3-Gal8-P2A-Zeo was a gift from Jordan Green (Addgene plasmid # 150815 ; ; RRID:Addgene_150815)
  • For your References section:

    High-throughput and high-content bioassay enables tuning of polyester nanoparticles for cellular uptake, endosomal escape, and systemic in vivo delivery of mRNA. Rui Y, Wilson DR, Tzeng SY, Yamagata HM, Sudhakar D, Conge M, Berlinicke CA, Zack DJ, Tuesca A, Green JJ. Sci Adv. 2022 Jan 7;8(1):eabk2855. doi: 10.1126/sciadv.abk2855. 10.1126/sciadv.abk2855 PubMed 34985952