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pCL20C-ddIPpoI
(Plasmid #49053)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49053 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCL20c
  • Total vector size (bp) 9014
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DD-HA-ER-IPpoI
  • Species
    H. sapiens (human); Physarum polycephalum
  • Insert Size (bp)
    1826

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SgrAI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCL20C-ddIPpoI was a gift from Michael Kastan (Addgene plasmid # 49053 ; http://n2t.net/addgene:49053 ; RRID:Addgene_49053)
  • For your References section:

    Nucleolin mediates nucleosome disruption critical for DNA double-strand break repair. Goldstein M, Derheimer FA, Tait-Mulder J, Kastan MB. Proc Natl Acad Sci U S A. 2013 Sep 30. 10.1073/pnas.1306160110 PubMed 24082117