161,813 results

1. pDONR221-Neo

   Plasmid

   #48993

   Purpose

   Gateway adapted transfer of Neomycin resistance gene

   Depositor

   Rick Tarleton

   Article

   Xu et al BMC Microbiol. 2009 May 11;9:90. doi: 10

   Insert

   aminoglycoside 3' phosphotransferase
   

   Use

   Gateway attb1 & attb2

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCMV 3xFLAG-LC3B WT

   Plasmid

   #123092

   Purpose

   Expresses 3xFLAG-LC3B wild-type in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. control (pGS701)

   Plasmid

   #160037

   Purpose

   pLV-A4-UCOE-EF1-3xHA-GFP-MCS-IRES-BLAS-2A-SNAP-WPRE-A2

   Depositor

   David Sabatini

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-Rab4B

   Plasmid

   #61801

   Purpose

   Human Rab4B fluorescently tagged with GFP on the N-terminus for mammalian expression

   Depositor

   Gia Voeltz

   Article

   Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

   Insert

   Rab4B (RAB4B Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   Jan. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. Anti-Histone H3.3 [N158/28R]

   Plasmid

   #182106

   Purpose

   Mammalian Expression Plasmid of anti-Histone H3.3 (Rat). Derived from hybridoma N158/28.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-Histone H3.3 (Rattus norvegicus) recombinant mouse monoclonal antibody (H3f3b Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF1152

   Plasmid

   #142739

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   MYOCD (MYOCD Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF0727

   Plasmid

   #141674

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZFP36L2 (ZFP36L2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV BiP-Myc-KDEL-wt

   Plasmid

   #27164

   Purpose

   Mammalian expression of human Bip with Myc tag near the C-terminus, retaining the C-terminal KDEL ER localization sequence

   Depositor

   Ron Prywes

   Article

   Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

   Insert

   BiP (HSPA5 Human)
   

   Use

   Tags

   KDEL and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWXC078

   Plasmid

   #222961

   Purpose

   C. elegans codon-optimized mScarlet-I3 with 3 introns cloned into pPD95.75 backbone

   Depositor

   Oliver Hobert

   Article

   Cao et al Genetics. 2024 Oct 7;228(2):iyae126. doi

   Insert

   mScarlet-I3
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-hTipin (pc4732)

    Plasmid

    #216862

    Purpose

    Mammalian expression vector encoding GFP tagged human Tipin

    Depositor

    Cristina Cardoso

    Article

    Prorok et al Front Cell Dev Biol. 2024 Feb 29;12:1346

    Insert

    Tipin (TIPIN Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKA-207I10

    Plasmid

    #79845

    Purpose

    Expresses NLS-PpsR2-VP16-IRES-RpBphP1-mCherry-TetR under CMV promoter

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

    Insert

    NLS-PpsR2-VP16-IRESI10-BphP1-mCherry-TetR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    RpPpsR2 cysteine 439 changed to serine

    Promoter

    CMV

    Available since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST-CMV-C-EGFP

    Plasmid

    #122844

    Purpose

    C-terminal EGFP tag. Gateway destination vector for mammalian expression.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-TadA8e-Ns3h

    Plasmid

    #221227

    Purpose

    Express HACE Editor as a fusion of TadA-8e adenosine deaminase and Ns3h helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    TadA8e-Ns3h
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-Ftractin-mRuby3-p2A-mTurquoise-MLC-IRES-Blast

    Plasmid

    #85146

    Purpose

    lentiviral expression of a stoichiometric F-actin and myosin light chain reporter

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Insert

    Ftractin-mRuby3-P2A-mTurquoise-MLC
    

    Use

    Lentiviral

    Tags

    mRuby3, mTurquoise

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-EF1aL-eGFP-W

    Plasmid

    #126686

    Purpose

    Constitutive, ubiquitous GFP expression.

    Depositor

    Darrell Kotton

    Article

    Jacob et al Nat Protoc. 2019 Dec;14(12):3303-3332. d

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1aL

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKEW302-AcrVA4

    Plasmid

    #115656

    Purpose

    Expresses AcrVA4 in bacteria, under TetR control.

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    AcrVA4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. secretedBirA-8his

    Plasmid

    #32408

    Depositor

    Gavin Wright

    Article

    Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

    Insert

    BirA
    

    Use

    Tags

    8 His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. lenti-CDDR

    Plasmid

    #149355

    Purpose

    CRISPR/Cas9-based dual-fluorescent DSB repair reporter

    Depositor

    Tarek Abbas

    Article

    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

    Inserts

    CDDR Reporter
    Blasticidin resistance
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a and hPGK

    Available since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-28a+GFP-CAMSAP3 CKK

    Plasmid

    #59033

    Purpose

    expresses CKK domain of CAMSAP3 in E. coli

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    CAMSAP3 CKK domain (CAMSAP3 Mouse)
    

    Use

    Tags

    His, Strep, TEV, and sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pOTIII.EFGP.IGR3.6

    Plasmid

    #40866

    Depositor

    Harold Gainer

    Article

    Fields et al J Neurosci. 2003 Aug 27;23(21):7801-9.

    Insert

    MOUSE OXYTOCIN GENE (Oxt Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    mouse oxytocin promoter

    Available since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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