FR_HNF6
(Plasmid #31099)

• Depositing Lab: Gerhart Ryffel
• Publication: Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

Backbone

• Vector backbone: pcDNA5/FRT/TO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5150
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HNF6
• Alt name: ONECUT
• Species: H. sapiens (human)
• Insert Size (bp): 1450
• GenBank ID: NM_004498
• Entrez Gene: ONECUT1 (a.k.a. HNF-6, HNF6, HNF6A)
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: na

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

for Flp mediated site directed integration, tetracycline inducibilty