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Optogenetic control with a photocleavable protein, PhoCl.
Zhang W, Lohman AW, Zhuravlova Y, Lu X, Wiens MD, Hoi H, Yaganoglu S, Mohr MA, Kitova EN, Klassen JS, Pantazis P, Thompson RJ, Campbell RE
Nat Methods. 2017 Mar 13. doi: 10.1038/nmeth.4222.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
87688pBAD/HisB-PhoClExpress PhoCl in E. coli
87689pBAD/HisB-PhoCl-MBPExpress PhoCl-MBP in E.coli
87690pcDNA-NES-PhoCl-mCherryExpress NES-PhoCl-mCherry in mammalian cells
87691pcDNA-NLS-PhoCl-mCherryExpress NLS-PhoCl-mCherry in mammalian cells
87692pcDNA-PhoCl-mCherry-mycExpress PhoCl-mCherry with C-term myc tag in mammalian cells
87693pCAG-ERT2-PhoCl-Gal4VP16-PhoCl-ERT2Express ERT2-PhoCl-Gal4VP16-PhoCl-ERT2 in mammalian cells
87694pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2Express ERT2-PhoCl-Cre-PhoCl-ERT2 in mammalian cells
87695pUAS-mCherry-NLSGal4VP16 driven expression of mCherry-NLS in mammalian cells
87696pUAS-NanoLucGal4VP16 driven expression of NanoLuc
87697pcDNA-I-PhoCl-HCVpExpress I-PhoCl-HCVp with N-term EBFP2 in mammalian cells
87698pcDNA-PANX1(HCVp)Express HCV protease-activatible pannexin-1 in mammalian cells
87699pcDNA-mCherry-4A/4B-CAAXExpress mCherry with a peptide linker (contaning a HCVp 4A/4B substrate site) and a C-terminal CAAX box from KRras for plasma-membrane targeting in mammalian cells

Antibodies from Article