PurposeExpress NES-PhoCl-mCherry in mammalian cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||87690||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
- Promoter CMV
/ Fusion Protein
- nuclear export signal (N terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA-NES-PhoCl-mCherry was a gift from Robert Campbell (Addgene plasmid # 87690 ; http://n2t.net/addgene:87690 ; RRID:Addgene_87690)
For your References section:Optogenetic control with a photocleavable protein, PhoCl. Zhang W, Lohman AW, Zhuravlova Y, Lu X, Wiens MD, Hoi H, Yaganoglu S, Mohr MA, Kitova EN, Klassen JS, Pantazis P, Thompson RJ, Campbell RE. Nat Methods. 2017 Mar 13. doi: 10.1038/nmeth.4222. 10.1038/nmeth.4222 PubMed 28288123