PurposeExpress ERT2-PhoCl-Cre-PhoCl-ERT2 in mammalian cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||87694||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Growth Strain(s)NEB Stable
- Promoter Chicken beta actin
- Cloning method Unknown
- 5′ sequencing primer pCAG-F (Common Sequencing Primers)
There's a Leu to Val mismatch between the reference sequence and Addgene's quality control results. The change is in the linker region between PhoCl and ERT2 and should not affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2 was a gift from Robert Campbell (Addgene plasmid # 87694 ; http://n2t.net/addgene:87694 ; RRID:Addgene_87694)
For your References section:Optogenetic control with a photocleavable protein, PhoCl. Zhang W, Lohman AW, Zhuravlova Y, Lu X, Wiens MD, Hoi H, Yaganoglu S, Mohr MA, Kitova EN, Klassen JS, Pantazis P, Thompson RJ, Campbell RE. Nat Methods. 2017 Mar 13. doi: 10.1038/nmeth.4222. 10.1038/nmeth.4222 PubMed 28288123