A set of powerful negative selection systems for unmodified Enterobacteriaceae.
Khetrapal V, Mehershahi K, Rafee S, Chen S, Lim CL, Chen SL
Nucleic Acids Res. 2015 Jul 27;43(13):e83. doi: 10.1093/nar/gkv248. Epub 2015 Mar 23. PubMed Article
Khetrapal V, Mehershahi K, Rafee S, Chen S, Lim CL, Chen SL
Nucleic Acids Res. 2015 Jul 27;43(13):e83. doi: 10.1093/nar/gkv248. Epub 2015 Mar 23. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 73163 | pSLC-217 | Template plasmid which encodes a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) to be used as a negative selection marker. |
| 73189 | pSLC-242 | Template plasmid which encodes chloramphenicol resistance gene for positive selection and a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) for negative selection. |
| 73190 | pSLC-246 | Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (tse2) under the control of rhamnose induceable promoter (PrhaB) for negative selection. |
| 73191 | pSLC-237 | Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (relE) under the control of arabinose induceable promoter (ParaB) for negative selection. |
| 73192 | pSLC-238 | Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (chpB) under the control of rhamnose induceable promoter (PrhaB) for negative selection. |
| 73193 | pSLC-239 | Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (yhaV) under the control of rhamnose induceable promoter (PrhaB) for negative selection. |
| 73194 | pSLC-241 | Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (mqsR) under the control of rhamnose induceable promoter (PrhaB) for negative selection. |