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PurposeLive-cell marker for stable microtubules
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 174649 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneB-actin
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehKif5B(1-560)rigor-2xmNeongreen
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SpeciesH. sapiens (human)
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Insert Size (bp)3156
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Mutationrigor mutation G234A
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Entrez GeneKIF5B (a.k.a. HEL-S-61, KINH, KNS, KNS1, UKHC)
- Promoter Chicken beta-actin
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Tag
/ Fusion Protein
- mNeongreen-mNeongreen (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Internal plasmid ID: KJ116
Please visit https://www.biorxiv.org/content/10.1101/2021.06.23.449589v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pB80-hKif5b(1-560)rigor-2xmNeongreen was a gift from Lukas Kapitein (Addgene plasmid # 174649 ; http://n2t.net/addgene:174649 ; RRID:Addgene_174649) -
For your References section:
A live-cell marker to visualize the dynamics of stable microtubules throughout the cell cycle. Jansen KI, Iwanski MK, Burute M, Kapitein LC. J Cell Biol. 2023 May 1;222(5):e202106105. doi: 10.1083/jcb.202106105. Epub 2023 Mar 2. 10.1083/jcb.202106105 PubMed 36880745
