Addgene: TRUPATH, an open-source biosensor platform for interrogating the GPCR transducerome.

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Bryan Roth
Olsen et al

TRUPATH, an open-source biosensor platform for interrogating the GPCR transducerome.
Olsen RHJ, DiBerto JF, English JG, Glaudin AM, Krumm BE, Slocum ST, Che T, Gavin AC, McCorvy JD, Roth BL, Strachan RT
Nat Chem Biol. 2020 May 4. pii: 10.1038/s41589-020-0535-8. doi: 10.1038/s41589-020-0535-8. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
140973	pcDNA5/FRT/TO-GAlphai1-RLuc8	Encodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation	Loading...
140974	pcDNA5/FRT/TO-GAlphai2-RLuc8	Encodes a G alpha subunit (GNAI2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation	Loading...
140975	pcDNA5/FRT/TO-GAlphai3-RLuc8	Encodes a G alpha subunit (GNAI3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation	Loading...
140976	pcDNA5/FRT/TO-GAlphaoA-RLuc8	Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation	Loading...
140977	pcDNA5/FRT/TO-GAlphaoB-RLuc8	Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation	Loading...
140978	pcDNA5/FRT/TO-GAlphaz-RLuc8	Encodes a G alpha subunit (GNAZ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation	Loading...
140979	pcDNA5/FRT/TO-GAlphaGustducin-RLuc8	Encodes a G alpha subunit (GNAT3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation	Loading...
140980	pcDNA5/FRT/TO-GAlphasS-RLuc8	Encodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation	Loading...
140981	pcDNA5/FRT/TO-GAlphasL-RLuc8	Encodes a G alpha subunit (GNAS1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation	Loading...
140982	pcDNA5/FRT/TO-GAlphaQ-RLuc8	Encodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation	Loading...
140983	pcDNA5/FRT/TO-GAlpha11-RLuc8	Encodes a G alpha subunit (GNA11) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation	Loading...
140984	pcDNA5/FRT/TO-GAlpha15-RLuc8	Encodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation	Loading...
140985	pcDNA5/FRT/TO-GAlpha12-RLuc8	Encodes a G alpha subunit (GNA12) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation	Loading...
140986	pcDNA5/FRT/TO-GAlpha13-RLuc8	Encodes a G alpha subunit (GNA13) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation	Loading...
140987	pcDNA3.1-Beta1	Encodes a Gbeta subunit (GNB1) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation	Loading...
140988	pcDNA3.1-Beta3	Encodes a Gbeta subunit (GNB3) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation	Loading...
140989	pcDNA3.1-GGamma1-GFP2	Encodes a G gamma subunit (GNGT1) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation	Loading...
140990	pcDNA3.1-GGamma8-GFP2	Encodes a G gamma subunit (GNG8) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation	Loading...
140991	pcDNA3.1-GGamma9-GFP2	Encodes a G gamma subunit (GNG9) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation	Loading...
140992	pcDNA3.1-GGamma13-GFP2	Encodes a G gamma subunit (GNG13) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation	Loading...
166772	pcDNA3.1-Beta2	Encodes a Gbeta subunit as a "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation	Loading...
166773	pcDNA3.1-Beta4	Encodes a Gbeta subunit as a "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation	Loading...
166774	pcDNA3.1-GGamma2-GFP2	Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation	Loading...
166775	pcDNA3.1-GGamma3-GFP2	Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation	Loading...
166776	pcDNA3.1-GGamma4-GFP2	Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation	Loading...
166777	pcDNA3.1-GGamma5-GFP2	Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation	Loading...
166778	pcDNA3.1-GGamma7-GFP2	Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation	Loading...
166779	pcDNA3.1-GGamma10-GFP2	Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation	Loading...
166780	pcDNA3.1-GGamma11-GFP2	Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation	Loading...
166781	pcDNA3.1-GGamma12-GFP2	Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation	Loading...

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