161,709 results

1. L4440_BioBrick-hif-1-sgRNA-B

   Plasmid

   #177785

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   hif-1 (hif-1 Nematode)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Td-CGBE

   Plasmid

   #196599

   Purpose

   Mammalian expression of Td-CGBE

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

   Insert

   bpNLS-TadA-8e (N46L)-32aa linker-nSpCas9-bpNLS
   

   Use

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   TadA-8e (N46L); S. pyogenes Cas9 (D10A)

   Promoter

   Available since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Flag-M4-AR

   Plasmid

   #171240

   Purpose

   Mammalian expression of 3xFlag-tagged human androgen receptor (hAR)

   Depositor

   Steven Balk

   Article

   Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

   Insert

   3xFlag-tagged human androgen receptor (AR Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. DPYSL2-pLJC2

   Plasmid

   #189866

   Purpose

   Expresses human DPYSL2 (CRMP2) in mammalian cells

   Depositor

   Yoav Shaul

   Article

   Abu Rmaileh et al J Cell Biol. 2022 Jul 4;221(7). pii: 213

   Insert

   DPYSL2 (DPYSL2 Human)
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFRT_TO_FlagHA_USP10

   Plasmid

   #127102

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   USP10 (USP10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. HA-APP-mGFP

   Plasmid

   #196696

   Purpose

   Expresses APP695 with an HA tag an N-terminal for single molecule tracking and other measusers in mammalian cells

   Depositor

   Martino Calamai

   Article

   Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

   Insert

   Amyloid Precursor Protein 695 isoform (APP Human)
   

   Use

   Tags

   HA and mGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3-Flag MKK6

   Plasmid

   #13517

   Depositor

   Roger Davis

   Article

   Raingeaud et al Mol Cell Biol. 1996 Mar . 16(3):1247-55.

   Insert

   MKK6 (MAP2K6 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 25, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pWZL hygro Flag HA TRAP220 wt

   Plasmid

   #17433

   Purpose

   Retroviral expression of Med1/TRAP220 in mammalian cells

   Depositor

   Kai Ge

   Article

   Ge et al Mol Cell Biol. 2008 Feb . 28(3):1081-91.

   Insert

   TRAP220 (MED1 Human)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLY100-RetroEFS-HER2-28BBz-WPRE

   Plasmid

   #192201

   Purpose

   Retro-HER2CAR

   Depositor

   Sidi Chen

   Article

   Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

   Insert

   Retro-HER2CAR (ERBB2 Synthetic)
   

   Use

   Retroviral; Mammalian expression

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSET.HaloTag.v7.AviTag

    Plasmid

    #195486

    Purpose

    Bacterial expression plasmid with Halotag v7 insert plus a C-terminus appended AviTag for enzymatic biotinylation.

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Insert

    HaloTag.v7.AviTag
    

    Use

    Tags

    6xHIS and AviTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. sgOpti

    Plasmid

    #85681

    Purpose

    Lentiviral vector to express sgRNAs. The cloning site is BsmBI.

    Depositor

    David Sabatini, Eric Lander

    Article

    Fulco et al Science. 2016 Sep 29. pii: aag2445.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBITE-EGFRvIII-F269-OKT3

    Plasmid

    #190681

    Purpose

    Encodes an EGFRvIII-CD3 BITE protein (F269 clone)

    Depositor

    Scott McComb

    Article

    Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

    Insert

    Anti-EGFRvIII-scFv-F269-modularized-OKT3-HisTag
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLDPuro-hsnSR100N

    Plasmid

    #35172

    Depositor

    Benjamin Blencowe

    Article

    Raj et al Mol Cell. 2011 Sep 2;43(5):843-50.

    Insert

    SRRM4 (SRRM4 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEMHE-mCherry-mTrim21

    Plasmid

    #105522

    Purpose

    T7 promotor drives in vitro transcription of mCherry-tagged wildtype mouse TRIM21 mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    TRIM21 (Trim21 Mouse)
    

    Use

    In vitro transcription

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CMVp-dsRed2-Triplex-HHRibo-gRNA1-HDVRibo-pA

    Plasmid

    #55201

    Purpose

    Plasmid encoding the Ribozyme/gRNA architecture. This is a modified form of the original plasmid described in the paper (Construct 13). mKate2 was replaced with dsRed2 because of distribution issues.

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    dsRed2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. UKNP1.18.1

    Plasmid

    #98190

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti_rtTA-P2A-BFP_HygroR

    Plasmid

    #186738

    Purpose

    rtTA-P2A-BFP expression construct cloned into lentiviral backbone

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Insert

    rtTA
    

    Use

    Lentiviral

    Tags

    P2A-BFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. LM670

    Plasmid

    #191552

    Purpose

    LM670_MSG-AGGA-promoter-ccdb-TTCC-GShis

    Depositor

    David Baker

    Article

    Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

    Insert

    ccdb
    

    Use

    Tags

    HIS-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMBP-FnCas12a

    Plasmid

    #113432

    Purpose

    Expresses 10xHis-MBP fused to FnCas12a

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    FnCas12a
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP-coilin

    Plasmid

    #36906

    Depositor

    Greg Matera

    Article

    Shpargel et al J Cell Sci. 2003 Jan 15;116(Pt 2):303-12

    Insert

    coilin (COIL Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge