166,034 results

1. pCMV-PEmax-P2A-hP53DD-P2A-hMLH1dn

   Plasmid

   #214102

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and P2A-human MLH1dn

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   PE-Plus
   

   Expression

   Mammalian

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pINDUCER20-Tau

   Plasmid

   #92201

   Purpose

   Retroviral overexpression vector (doxycycline-inducible) for Tau

   Depositor

   Huda Zoghbi

   Article

   Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

   Insert

   MAPT (MAPT Human)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCLIP-tag (m) Vector

   Plasmid

   #101136

   Purpose

   Expression of protein of interest with CLIP-Tag in Mammalian cells

   Depositor

   New England Biolabs, Ana Egana

   Type

   Empty backbone

   Tags

   CLIP-tag (CLIP10)

   Expression

   Mammalian

   Available Since

   Sept. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. SARS-CoV-2 S HexaPro

   Plasmid

   #154754

   Purpose

   Mammalian expression vector for expression of the SARS-CoV-2 spike HexaPro variant

   Depositor

   Jason McLellan

   Article

   Hsieh et al Science. 2020 Sep 18;369(6510):1501-1505

   Insert

   Spike (HexaPro variant) (S Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …

   Expression

   Mammalian

   Mutation

   Ectodomain only (AAs 1-1208); 682-685 (furin site…

   Available Since

   July 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. piggyBac-rtTA (4th_Gen)-SNCA(3K)-sfGFP-IRES-NGN2-puro (VK_1120)

   Plasmid

   #209078

   Purpose

   Ngn2 mediated cortical neuron induction, along with SNCA(3K) over-expression

   Depositor

   Vikram Khurana

   Article

   Lam et al bioRxiv 2022.11.08.515615

   Inserts

   SNCA (SNCA Human)
   NGN2 (Neurog2 Mouse)
   

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   3K mutant: E35K, E46K, E61K

   Promoter

   TRE4G

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-FLAG-MTOR-E1799K

   Plasmid

   #69009

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR-E1799K (MTOR Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   change Glutamate 1799 to Lysine

   Available Since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC gRNA Shuttle

   Plasmid

   #47024

   Purpose

   Encodes a template from which gRNAs can be made via InFusion cloning. The Medicago truncatula U6.6 promoter drives the gRNA. For use in plants.

   Depositor

   Wayne Parrott

   Article

   Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

   Insert

   gRNA Shuttle
   

   Use

   CRISPR; Cas9

   Expression

   Plant

   Mutation

   G to T cloning mutation at position 323

   Promoter

   Medicago truncatula U6.6

   Available Since

   Sept. 3, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. qTAG-AAVS1-PGK-Zeo

   Plasmid

   #227271

   Purpose

   Empty AAVS1 targeting donor for the insertion of Zeo and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-TRE-HTG

   Plasmid

   #27437

   Depositor

   Liqun Luo

   Article

   Miyamichi et al Nature. 2011 Apr 14;472(7342):191-6. doi

   Insert

   histone-GFP-2A-TVA receptor-2A-rabies glycoprotein
   

   Use

   AAV; Adeno-associated viral

   Mutation

   N/A

   Available Since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. CMV-mEGFP(A206K)-paCaMKII(K42M)

    Plasmid

    #165440

    Purpose

    Expresses photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    mEGFP(A206K)-paCaMKII(K42M)
    

    Tags

    mEGFP(A206K)

    Expression

    Mammalian

    Mutation

    K42M: Kinase dead mutation

    Promoter

    CMV

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. N-cadherin in pCCL-c-MNDU3c-PGK-EGFP

    Plasmid

    #38153

    Depositor

    Nora Heisterkamp

    Article

    Zhang et al Leukemia. 2007 Jun;21(6):1189-97. Epub 2

    Inserts

    N-cadherin (CDH2 Human)
    EGFP
    

    Use

    Lentiviral

    Promoter

    MNDU3c = LTR promoter for U3 region of myeloproli…

    Available Since

    Aug. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. A*24:02:01

    Plasmid

    #203295

    Purpose

    Overexpress HLA allele

    Depositor

    Derin Keskin, Catherine Wu

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*24:02:01 (HLA-A Human)
    

    Expression

    Mammalian

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. PIK3CA-WT

    Plasmid

    #16643

    Depositor

    Bert Vogelstein

    Article

    Samuels et al Science. 2004 Apr 23. 304(5670):554.

    Insert

    PIK3CA (PIK3CA Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1 - TRC mTagBFP2

    Plasmid

    #191566

    Purpose

    Expresses an shRNA and mTagBFP2

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6 for shRNA

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-PTEN-C124S

    Plasmid

    #28300

    Depositor

    Maria-Magdalena Georgescu

    Article

    Takahashi et al EMBO J. 2006 Feb 22. 25(4):910-20.

    Insert

    PTEN (PTEN Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Phosphatase Inactive Mutant Cys124Ser

    Available Since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3 IKKe flag

    Plasmid

    #26201

    Depositor

    Tom Maniatis

    Article

    Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

    Insert

    I-kappa-B kinase epsilon (IKBKE Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR223-BORF2

    Plasmid

    #195834

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BORF2

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BORF2
    

    Use

    Gateway entry vector

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. miniTurbo-V5_pRS415

    Plasmid

    #107168

    Purpose

    expresses V5-tagged miniTurbo in the yeast cytosol

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Expression

    Yeast

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. All-in-one lenti-ABE8e-NG

    Plasmid

    #200447

    Purpose

    Lentivirus vector for base editing

    Depositor

    Benjamin Cravatt

    Article

    Li et al Nat Chem Biol. 2023 Nov;19(11):1320-1330

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBMN-2xFKBP-mEGFP-NDP52

    Plasmid

    #110465

    Purpose

    retrovirus construct for stable expressing mEGFP tagged NDP52

    Depositor

    Richard Youle

    Article

    Lazarou et al Nature. 2015 Aug 20;524(7565):309-14. do

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Retroviral; Retrovirus

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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