163,606 results

1. pcDNA3/Flag-METTL14

   Plasmid

   #53740

   Purpose

   For expression of METTL14 (methyltransferase-like 14) in human cells

   Depositor

   Chuan He

   Article

   Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

   Insert

   Methyltransferase-like 14 (METTL14 Human)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCS2-nCas9n-nanos 3’UTR

   Plasmid

   #62542

   Purpose

   expression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafish

   Depositor

   Antonio Giraldez

   Article

   Moreno-Mateos et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

   Insert

   nanos 3' UTR (nanos3 Zebrafish)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   3' UTR only

   Promoter

   SP6

   Available Since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. plentiCMV-sct-p5-b2m-HLA-A0101

   Plasmid

   #196512

   Purpose

   Express single-chain peptide-MHC (CMV p5 epitope on HLA-A0101)

   Depositor

   Howard Chang

   Article

   Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

   Insert

   single chain of a CMV peptide, b2m and HLA A0101
   

   Use

   Lentiviral

   Tags

   NA

   Expression

   Mammalian

   Mutation

   Y84C

   Promoter

   CMV

   Available Since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCDFDuet-MKK6-EE p38alpha

   Plasmid

   #82719

   Purpose

   Expresses constitutively active human MKK6 and murine p38 alpha.

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Inserts

   MKK6 (MAP2K6 Human)
   p38alpha (Mapk14 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Ser207 to Glu, Thr211 to Glu

   Promoter

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

5. pLCNICK

   Plasmid

   #84653

   Purpose

   Genome editing for Lactobacillus casei Lc2W

   Depositor

   Sheng Yang

   Article

   Song et al Appl Environ Microbiol. 2017 Sep 1. pii:

   Inserts

   Cas9 nickase
   sgRNA
   homology arms of LC2W_2179
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A

   Promoter

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mVenus C1

   Plasmid

   #27794

   Depositor

   Steven Vogel

   Article

   Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

   Has Service

   Cloning Grade DNA

   Insert

   mVenus C1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pScalps_Puro_mCebpa

   Plasmid

   #79551

   Purpose

   Expression of mouse Cebpa

   Depositor

   Silvia Monticelli

   Article

   Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

   Insert

   Cebp alpha (Cebpa Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET30b-7+-SrtA

   Plasmid

   #105602

   Purpose

   expressed 7+ sortase A variant in E.coli

   Depositor

   Stephanie Dougan

   Article

   Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

   Insert

   S.aureus Sortase A, 7+ mutant
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Fuw-dCas9-Dnmt3a-P2A-tagBFP

   Plasmid

   #84569

   Purpose

   Lentiviral construct to express dCas9 fused with Dnmt3a-P2A-tagBFP

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

   Insert

   dCas9-Dnmt3a (DNMT3A Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCFD5_w

    Plasmid

    #112645

    Purpose

    Ubiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.

    Depositor

    Michael Boutros

    Insert

    U6:3-tRNA-sgRNA expression cassette
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti6/V5-DEST-NCAPH

    Plasmid

    #31214

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    NCAPH (NCAPH Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV_Xph15-GCaMP6f

    Plasmid

    #135538

    Purpose

    Encodes a specific PSD-95 binder (Xph15) fused to GCaMP6f

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph15
    

    Use

    Tags

    GCaMP6f

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFB2B

    Plasmid

    #195284

    Purpose

    Bicistronic HSV-TK and Puromycin resistance for positive/negative selection

    Depositor

    Scott Lowe

    Article

    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

    Insert

    HSV-TK 2A Puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pScaf-3024.1

    Plasmid

    #111404

    Purpose

    pScaf phagemid with insert for generating DNA origami scaffold 3024 bases in length

    Depositor

    Shawn Douglas

    Article

    Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

    Insert

    pScaf-3024
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLPCX mito roGFP2-Orp1

    Plasmid

    #64992

    Purpose

    Mammalian expression of mitochondrial roGFP2-Orp1 (retroviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Retroviral

    Tags

    roGFP2

    Expression

    Mammalian

    Mutation

    mitochondrial targeting sequence

    Promoter

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. plenti.CamKII.(synapto).cpsfGFP.miRFP670nano3

    Plasmid

    #214926

    Purpose

    Expresses presynaptic non-responsive control

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (synapto).cpsfGFP.miRFP670nano3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. P307-FBP_6

    Plasmid

    #139697

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    various

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. UAS-mCherry

    Plasmid

    #135457

    Purpose

    Mammalian mCherry reporter to read out FLARE and SPARK activation

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    UAS

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-flag YAP2 5SA

    Plasmid

    #27371

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    YAP2 (YAP1 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Serines at positions 61, 109, 127, 128, 131, 163,…

    Promoter

    Available Since

    Feb. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-28a-mCherry-MED1-IDR

    Plasmid

    #194545

    Purpose

    Bacterial Expression of 6xHis mCherry-MED1-IDR Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    MED1 (MED1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge