176,161 results

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pFL-SV40

Plasmid

#115352

Purpose

firefly luciferase (Fluc) reporter gene driven by SV40 promoter

Depositor

Ming-Chih Lai

Publication

Lai et al Mol Biol Cell. 2008 Sep;19(9):3847-58. d

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGPA1

Plasmid

#115476

Purpose

Generation of mariner-based transposon mutant libraries in Enterococcus faecium

Depositor

Willem van Schaik

Publication

Zhang et al BMC Genomics. 2017 Nov 21;18(1):893. doi

Type

Empty backbone

Expression

Bacterial

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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7a sgRNA for EJ7-GFP and 4-μHOM reporters

Plasmid

#113620

Purpose

sgRNA/CAS9 expression plasmid to induce the 5’ double-strand break in both the EJ7-GFP and 4-μHOM reporters

Depositor

Jeremy Stark

Publication

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7a sgRNA

Use

CRISPR

Expression

Mammalian

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles
EasyFusion Halo

Plasmid

#112850

Purpose

General donor vector with HaloTag coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

HaloTag

Use

Synthetic Biology

Promoter

No

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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AAV-P(Per2)-DIO-intron2-dLUC

Plasmid

#110059

Purpose

Per2 transcription luciferase reporter

Depositor

Eric Erquan Zhang

Publication

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Per2 promoter and luciferase (Per2 Mouse)

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

Per2

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CamKII.GCaMP6f.WPRE.SV40

Plasmid

#100834

Purpose

AAV expression of ultrasensitive protein calcium sensor from CamKII promoter

Depositor

James M. Wilson

Has service

AAV1, AAV5, and AAV9

Insert

GCaMP6f

Use

AAV

Expression

Mammalian

Mutation

GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

Promoter

CamKII

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

80 Articles

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pX-evoCas9

Plasmid

#107550

Purpose

Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells

Depositor

Anna Cereseto

Publication

Casini et al Nat Biotechnol. 2018 Jan 29. pii: nbt.40

Insert

Humanized S. pyogenes Cas9

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Mutation

M495V/Y515N/K526E/R661Q

Promoter

CBh

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHis-hTG2

Plasmid

#100719

Purpose

Expression of human transglutaminase 2 in E.coli

Depositor

Byung Il Lee

Publication

Han et al Int J Biol Macromol. 2010 Aug 1;47(2):19

Insert

TGM2 (TGM2 Human)

Tags

His

Expression

Bacterial

Mutation

silent mutation Arg580 (AGG--CGT) to enhance expr…

Promoter

T7

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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STARR-seq luciferase validation vector_ORI_neg.cont

Plasmid

#99315

Purpose

Negative control for luciferase validation vectors

Depositor

Alexander Stark

Publication

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Insert

neutral region; chr3: 55198740-55200041

Use

Luciferase; Starr-seq luciferase validation vector

Expression

Mammalian

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET28-his-3C_Tau352

Plasmid

#100092

Purpose

Expresses human full-length fetal tau protein with the N-terminal 3C-cleavable His6 tag

Depositor

Nikolai Sluchanko

Publication

Tugaeva et al PLoS One. 2017 Jun 2;12(6):e0178933. doi

Insert

Fetal microtubule-associated protein tau (MAPT Human)

Tags

His6

Expression

Bacterial

Mutation

wild type sequence

Promoter

T7

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-RUNX1-P2A-ERG

Plasmid

#97045

Purpose

Expresses RUNX1-P2A-ERG in mammalian cells. *Please see notes below on P2A cleaving efficiency.

Depositor

George Daley

Publication

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Runt Related Transcription Factor 1, ETS-Related Gene

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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lentiGuide-Hygro-iRFP670

Plasmid

#99377

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A iRFP670 from EF-1a promoter. 3rd generation lentiviral backbone.

Depositor

Kristen Brennand

Publication

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Inserts

S. pyogenes sgRNA cassette
Hygro-P2A-iRFP670

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a and hU6

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-hSyn1-mRuby2

Plasmid

#99126

Purpose

An AAV genome that expresses the fluorescent protein mRuby2 from the hSyn1 promoter

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mRuby2

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAAV-TRE-mRuby2

Plasmid

#99114

Purpose

An AAV genome with tet-inducible expression of the fluorescent protein mRuby2

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mRuby2

Use

AAV

Expression

Mammalian

Promoter

TRE

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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MSCV-human Erbb2-IRES-GFP

Plasmid

#91888

Purpose

Retroviral expression of human Erbb2

Depositor

Martine Roussel

Publication

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Erbb2 (ERBB2 Human)

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Promoter

MMLV gag

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEM-Cas9HF1-recA56

Plasmid

#89962

Purpose

Modified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.

Depositor

Michael Lynch

Publication

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Inserts

Cas9HF1
recA56

Expression

Bacterial

Mutation

N497A/R661A/Q695A/Q926A

Promoter

pTet and proD

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDicAID_nCas9-PmCDA_NptII_Della

Plasmid

#91694

Purpose

Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDella

Depositor

Akihiko Kondo

Publication

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Tags

PmCDA1

Expression

Plant

Mutation

D10A for nickase Cas9

Promoter

PcUbi

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA-I-PhoCl-HCVp

Plasmid

#87697

Purpose

Express I-PhoCl-HCVp with N-term EBFP2 in mammalian cells

Depositor

Robert Campbell

Publication

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

I-PhoCl-HCVp containing the Ki = 10.5 nM inhibitor (CP5-46A-4D5E)

Tags

EBFP

Expression

Mammalian

Promoter

CMV

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223_JAK2_WT

Plasmid

#81756

Purpose

Gateway Donor vector containing JAK2 , part of the Target Accelerator Plasmid Collection. *Please note this is not a true WT JAK2 sequence and contains a V617F mutation.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

JAK2 (JAK2 Human)

Use

Gateway Cloning

Mutation

V617F

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJH376

Plasmid

#86842

Purpose

mamalian expression of anti-CRISPR protein AcrIIA4

Depositor

Joseph Bondy-Denomy

Publication

Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

Insert

acrIIA4

Expression

Mammalian

Promoter

CMV

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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