Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25700||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 8377
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
SpeciesH. sapiens (human)
Insert Size (bp)926
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer AAAGTGAAAGTCGAGCTCGGTACC (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:HUW-TetO-hNanog was a gift from Rudolf Jaenisch (Addgene plasmid # 25700 ; http://n2t.net/addgene:25700 ; RRID:Addgene_25700)
For your References section:Direct cell reprogramming is a stochastic process amenable to acceleration. Hanna J, Saha K, Pando B, van Zon J, Lengner CJ, Creyghton MP, van Oudenaarden A, Jaenisch R. Nature. 2009 Dec 3. 462(7273):595-601. 10.1038/nature08592 PubMed 19898493