pCAG-delta90GFP
(Plasmid #26645)

• Depositing Lab: Anjen Chenn
• Publication: Wrobel et al Dev Biol. 2007 Sep 15. 309(2):285-97.

Backbone

• Vector backbone: pCAG
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: delta90 beta-catenin
• Alt name: beta catenin
• Species: M. musculus (mouse)
• Insert Size (bp): 2907
• Mutation: Deletion of first 90 aa of beta catenin
• Entrez Gene: Ctnnb1 (a.k.a. Bfc, Catnb, Mesc)
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pCAG_Fwd
• 3′ sequencing primer: EGFP-N

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry