Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pEF myc Gal cyclin T1 (1-726) (P#1026)
(Plasmid #14626)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14626 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    cyclin T1
  • Alt name
    cyclinT1
  • Alt name
    cycT1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2186
  • Entrez Gene
    CCNT1 (a.k.a. CCNT, CYCT1, HIVE1)
  • Tags / Fusion Proteins
    • myc (N terminal on backbone)
    • Gal (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer EF1a forward primer
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Full-length cyclin T1 fused to the DBD of Gal4(1–147) and inserted at the BamHI/XbaI sites of pEF-Bos-myc(EF). The cyclin insert contains H519R, S566P, P712S, and L724F compared to closest cyclin T1 reference sequence NP_001231.2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF myc Gal cyclin T1 (1-726) (P#1026) was a gift from Matija Peterlin (Addgene plasmid # 14626 ; http://n2t.net/addgene:14626 ; RRID:Addgene_14626)
  • For your References section:

    Interaction between P-TEFb and the C-terminal domain of RNA polymerase II activates transcriptional elongation from sites upstream or downstream of target genes. Taube R, Lin X, Irwin D, Fujinaga K, Peterlin BM. Mol Cell Biol. 2002 Jan . 22(1):321-31. 10.1128/MCB.22.1.321-331.2002 PubMed 11739744