pEF myc Gal cyclin T1 (1-726) (P#1026)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14626||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5500
Vector typeMammalian Expression
Growth in Bacteria
Gene/Insert namecyclin T1
SpeciesH. sapiens (human)
Insert Size (bp)2186
Entrez GeneCCNT1 (a.k.a. CCNT, CYCT1, HIVE1)
/ Fusion Proteins
- myc (N terminal on backbone)
- Gal (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer EF1a forward primer (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Full-length cyclin T1 fused to the DBD of Gal4(1–147) and inserted at the BamHI/XbaI sites of pEF-Bos-myc(EF). The cyclin insert contains H519R, S566P, P712S, and L724F compared to closest cyclin T1 reference sequence NP_001231.2.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEF myc Gal cyclin T1 (1-726) (P#1026) was a gift from Matija Peterlin (Addgene plasmid # 14626 ; http://n2t.net/addgene:14626 ; RRID:Addgene_14626)
For your References section:Interaction between P-TEFb and the C-terminal domain of RNA polymerase II activates transcriptional elongation from sites upstream or downstream of target genes. Taube R, Lin X, Irwin D, Fujinaga K, Peterlin BM. Mol Cell Biol. 2002 Jan . 22(1):321-31. 10.1128/MCB.22.1.321-331.2002 PubMed 11739744