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cfSGFP2-N
(Plasmid #37533)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 37533 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 4105
  • Total vector size (bp) 4825
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cysteine-free SGFP2-N
  • Alt name
    cfSGFP2-N
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    720
  • Mutation
    C48S and C70M mutations relative to SGFP2
  • Promoter CMV

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    cfSGFP2-N was a gift from Ikuo Wada (Addgene plasmid # 37533 ; http://n2t.net/addgene:37533 ; RRID:Addgene_37533)
  • For your References section:

    Development of cysteine-free fluorescent proteins for the oxidative environment. Suzuki T, Arai S, Takeuchi M, Sakurai C, Ebana H, Higashi T, Hashimoto H, Hatsuzawa K, Wada I. PLoS One. 2012;7(5):e37551. Epub 2012 May 23. 10.1371/journal.pone.0037551 PubMed 22649538