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pShuttle-FEN1hWT
(Plasmid #35027)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 35027 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pShuttle
  • Backbone manufacturer
    Stratgene
  • Backbone size w/o insert (bp) 7500
  • Total vector size (bp) 9000
  • Vector type
    Mammalian Expression, Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FEN1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1500
  • Entrez Gene
    FEN1 (a.k.a. FEN-1, MF1, RAD2)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site EcoRV (destroyed during cloning)
  • 5′ sequencing primer GGTctatataagcagagctg
  • 3′ sequencing primer CTGatcataatcagccataccac
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pShuttle-FEN1hWT was a gift from Sheila Stewart (Addgene plasmid # 35027 ; http://n2t.net/addgene:35027 ; RRID:Addgene_35027)
  • For your References section:

    FEN1 ensures telomere stability by facilitating replication fork re-initiation. Saharia A, Teasley DC, Duxin JP, Dao B, Chiappinelli KB, Stewart SA. J Biol Chem. 2010 Aug 27;285(35):27057-66. Epub 2010 Jun 15. 10.1074/jbc.M110.112276 PubMed 20551483