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Rapid derivation of genetically related mutants from embryonic cells harboring a recombinase-specific Trp53 platform.
Wei QX, Odell AF, van der Hoeven F, Hollstein M
Cell Cycle. 2011 Apr 15;10(8):1261-70. Epub 2011 Apr 15.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
46848pX1Vector modified from pBluescript SK(+) by adding more cloning sites
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46849pX21Gene targeting to generate Trp53 Platform (PLF) mouse
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46850pX28TOP RFP op. PGK-puro indicator plasmid to assay TOP IMCE efficiency
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46851pX41TOP Hupki-wt plasmid without selection marker
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46852pX61TOP Hupki-puro2A-Hupki-wt plasmid
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46854pX62TOP Hupki-puro2A-G245S plasmid to introduce mutant p53 to PLF ESC or MEFs by IMCE
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46857pX68OP Hupki-puro2A-A138V plasmid to indroduce mutant p53 to PLF ESC or MEFs by IMCE
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Recombinant Antibodies from Article