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Cumate-inducible gene expression system for sphingomonads and other Alphaproteobacteria.
Kaczmarczyk A, Vorholt JA, Francez-Charlot A
Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 10.1128/AEM.02296-13. Epub 2013 Aug 30.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
48094pQF-lacZCumate-inducible expression of lacZ
48095pQFCumate-inducible expression of 3xFLAG-tagged fusion protein
48096pQHCumate-inducible expression of HA-tagged fusion protein
48097pQMCumate-inducible expression of myc-tagged fusion protein
48098pQYCumate-inducible expression of SYFP2-tagged fusion protein
48099pQRCumate-inducible expression of mCherry-tagged fusion protein
48100pQCCumate-inducible expression of ECFP-tagged fusion protein
48101pQFDDestination plasmid (Gateway cloning) for cumate-inducible expression of 3xFLAG-tagged fusion protein
48102pQHDDestination plasmid (Gateway cloning) for cumate-inducible expression of HA-tagged fusion protein
48103pQMDDestination plasmid (Gateway cloning) for cumate-inducible expression of myc-tagged fusion protein
48104pQYDDestination plasmid (Gateway cloning) for cumate-inducible expression of SYFP2-tagged fusion protein
48107pAK501lacZ promoter-probe plasmid with pVS1 origin of replication and chloramphenicol resistance

Antibodies from Article