166,034 results

1. SIRT5 Flag

   Plasmid

   #13816

   Purpose

   Mammalian expression of human SIRT5 with flag tag

   Depositor

   Eric Verdin

   Article

   North et al Mol Cell. 2003 Feb . 11(2):437-44.

   Insert

   SIRT5 (SIRT5 Human)
   

   Tags

   flag

   Expression

   Mammalian

   Available Since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT3-EF1aH N90-beta-catenin

   Plasmid

   #86499

   Purpose

   Mammalian expression of Myc tagged N90 beta-catenin

   Depositor

   Xin Chen

   Article

   Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

   Insert

   beta-catenin (CTNNB1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 1-90; constitutively acti…

   Promoter

   EF1a

   Available Since

   Feb. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFRT_TO_FlagHA_G3BP1

   Plasmid

   #127106

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   G3BP1 (G3BP1 Human)
   

   Expression

   Mammalian

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSF4 TetCMV 5'TOP intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA

   Plasmid

   #104140

   Purpose

   Expresses TREAT-5'TOP reporter in mammalian cells

   Depositor

   Jeffrey Chao

   Article

   Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

   Insert

   Renilla luciferase
   

   Tags

   24xMS2 stem loops in 3'UTR, 24xPP7 stem loop…

   Expression

   Mammalian

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLexM-SLC3A2

   Plasmid

   #197994

   Purpose

   Expresses SLC3A2 as an N-terminal His tagged construct in mammalian cells

   Depositor

   Simon Newstead

   Article

   Parker et al Nat Commun. 2021 Dec 8;12(1):7147. doi:

   Insert

   SLC3A2 (SLC3A2 Human)
   

   Tags

   His tag

   Expression

   Mammalian

   Mutation

   Please see depositor comments below

   Promoter

   CMV

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. MS2-epegRNA-Dnmt1

   Plasmid

   #211372

   Purpose

   Dnmt1 targeting epegRNA with MS2 insertion in gRNA scaffold for v3 PE-eVLP production

   Depositor

   David Liu

   Article

   An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

   Insert

   MS2-epegRNA
   

   Expression

   Mammalian

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBR322-PVCpnf17-22

   Plasmid

   #198272

   Purpose

   Unmodified pPayload; contains WT PVC payloads/regulatory genes

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf17-22
   

   Expression

   Bacterial

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFA6a-mCherry-sfGFP-kanMX (pMaM17)

   Plasmid

   #173452

   Purpose

   Contains mCherry-sfGFP tandem fluorescent protein timer

   Depositor

   Anton Khmelinskii

   Article

   Khmelinskii et al Nat Biotechnol. 2012 Jun 24;30(7):708-14

   Insert

   mCherry-sfGFP-ADH1ter
   

   Expression

   Yeast

   Promoter

   no promoter

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-tdMCP-YFP

   Plasmid

   #99151

   Purpose

   Expresses dimeric MS2-coat binding protein tagged with YFP, 2nd generation lentiviral vector

   Depositor

   Ron Vale

   Article

   Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

   Insert

   Dimeric MS2-coat binding protein
   

   Use

   Lentiviral

   Tags

   eYFP

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJ609-dCas9-KR-puro

    Plasmid

    #158478

    Purpose

    Express nuclease dead Cas9 fused to KillerRed chromophore in mammalian cells

    Depositor

    Brendan Price

    Article

    House et al PLoS One. 2020 Dec 17;15(12):e0237759. d

    Insert

    dCas9-KR
    

    Use

    CRISPR

    Tags

    KillerRed

    Expression

    Mammalian

    Mutation

    Cas9m4 (D10A+D839A+H840A+N863A)

    Promoter

    CMV

    Available Since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-FLAG-MTOR-V2006I

    Plasmid

    #69012

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR (MTOR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    V2006I

    Promoter

    CMV

    Available Since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLI50

    Plasmid

    #13573

    Depositor

    Chia Lee

    Article

    Lee et al Gene. 1991 Jul 15. 103(1):101-5.

    Type

    Empty backbone

    Use

    S. aureus-e. coli shuttle vector

    Available Since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pet-15b-microID

    Plasmid

    #172881

    Purpose

    expresses microID in E. coli

    Depositor

    Julien Béthune

    Article

    Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

    Insert

    microID
    

    Tags

    myc

    Expression

    Bacterial

    Mutation

    fragment ([aa2-171]) of aquifex aeolicus BirA wit…

    Promoter

    T7

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Flag-FOXP3

    Plasmid

    #153147

    Purpose

    Expression of Flag tagged human FOXP3 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXP3 (FOXP3 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Galt1-Grx1-roGFP1-iE

    Plasmid

    #230029

    Purpose

    Expressing Grx1-roGFP1-iE fluorescent fusion protein in mammalian cells with localization in the Golgi apparatus.

    Depositor

    Pablo Rivera-Fuentes

    Insert

    Galt1-Grx1-roGFP1-iE (B4GALT1 Human, Synthetic)
    

    Expression

    Mammalian

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. myc EDC4 pCMVSport6

    Plasmid

    #86855

    Purpose

    mammalian expression of EDC4

    Depositor

    Sarah Slavoff

    Article

    D'Lima et al Nat Chem Biol. 2016 Dec 5. doi: 10.1038/

    Insert

    EDC4 (EDC4 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. CaMKAR

    Plasmid

    #205315

    Purpose

    Fluorescent Reporter for CaMKII Activity

    Depositor

    Mark Anderson, Elizabeth Luczak

    Article

    Reyes Gaido et al Sci Transl Med. 2023 Jun 21;15(701):eabq

    Insert

    CaMKAR
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. MSCV Myc T58A puro

    Plasmid

    #18773

    Depositor

    Scott Lowe

    Article

    Hemann et al Nature. 2005 Aug 11. 436(7052):807-11.

    Insert

    myc T58A (MYC Human)
    

    Use

    Retroviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    T58A

    Available Since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. Sfp pet29b C-terminal His Tag

    Plasmid

    #75015

    Purpose

    Expresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACP

    Depositor

    Michael Burkart

    Article

    Worthington et al Org Biomol Chem. 2006 Jan 7;4(1):44-6. E

    Insert

    Sfp
    

    Tags

    His Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE

    Plasmid

    #198516

    Purpose

    Expresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Cre-dependent recombination.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Has Service

    AAV1 and AAV5

    Insert

    PdCO
    

    Use

    AAV and Cre/Lox

    Tags

    Rho1D4 and mScarlet

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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