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Nucleosome assembly is required for nuclear pore complex assembly in mouse zygotes.
Inoue A, Zhang Y
Nat Struct Mol Biol. 2014 Jul;21(7):609-16. doi: 10.1038/nsmb.2839. Epub 2014 Jun 8.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
59745pcDNA-ELYS-emerin-EGFP-polyAExpress ELYS-emerin-EGFP fusion protein in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.
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59746pcDNA-EGFP-ELYS-polyAExpress EGFP-ELYS in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.
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59747pcDNA-emerin-EGFP-polyAExpress emerin-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.
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59748pcDNA-LBR-EGFP-polyAExpress LBR-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.
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59749pcDNA-LBR-mRFP1-polyAExpress LBR-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.
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59750pcDNA-RanWT-mRFP1-polyAExpress wild-type form of Ran-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.
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59762pcDNA-Flag-RCC1-polyAExpress Flag-tagged RCC1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.
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59780pcDNA-Flag-H3.3-polyAExpress Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.
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59781pcDNA-HIRA-polyAExpress H3.3 chaperone HIRA in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.
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59782pcDNA-HIRAmut-polyAThis Hira gene contains silent mutations in siRNA-targeted sequences (see Inoue and Zhang, 2014 NSMB).
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104560pcDNA-Ran-Q69L-mRFP1-polyAExpress Ran Q69L mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.
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104561pcDNA-Ran-T24N-mRFP1-polyAExpress Ran T24N mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.
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104562pcDNA-Nup37-EGFP-polyAExpress Nup37-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.
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Antibodies from Article