Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pcDNA3-Ii-OVA.I+II
(Plasmid #64596)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 64596 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Total vector size (bp) 7046
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ovalbumin
  • Species
    G. gallus (chicken)
  • Mutation
    Only amino acids 242-353 (class I and class II epitopes)
  • Promoter CMV
  • Tag / Fusion Protein
    • Invariant chain (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-Ii-OVA.I+II was a gift from Sandra Diebold & Martin Zenke (Addgene plasmid # 64596 ; http://n2t.net/addgene:64596 ; RRID:Addgene_64596)
  • For your References section:

    MHC class II presentation of endogenously expressed antigens by transfected dendritic cells. Diebold SS, Cotten M, Koch N, Zenke M. Gene Ther. 2001 Mar;8(6):487-93. 10.1038/sj.gt.3301433 PubMed 11313828