Purpose(Empty Backbone) Plant Gene Expression Vector
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||67583||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerStratagene, fragments of the vector were synthesized by GenArt-Life Technologies
- Backbone size (bp) 7081
Vector typePlant Expression
- Promoter M24
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer NOS-F (5'-GCGTTCAAAAGTCGCCTAAG-3')
- 3′ sequencing primer NOSterm-R (5'-attgccaaatgtttgaacga-3') (Common Sequencing Primers)
Terms and Licenses
The difference between this plasmid and pKDH (Addgene plasmid # 67584) is that pSIM24 KanR has a kanamycin resistance cassette where pKDH has a hygromycin resistance cassette. The kanamycin and hygromycin resistance cassettes are used for selection in plants.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSIM24 KanR was a gift from Indu Maiti (Addgene plasmid # 67583 ; http://n2t.net/addgene:67583 ; RRID:Addgene_67583)
For your References section:pSiM24 is a novel versatile gene expression vector for transient assays as well as stable expression of foreign genes in plants. Sahoo DK, Dey N, Maiti IB. PLoS One. 2014 Jun 4;9(6):e98988. doi: 10.1371/journal.pone.0098988. eCollection 2014. PONE-D-14-03371 [pii] PubMed 24897541