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pSIM24 KanR
(Plasmid #67583)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 67583 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    N/A
  • Backbone manufacturer
    Stratagene, fragments of the vector were synthesized by GenArt-Life Technologies
  • Backbone size (bp) 7081
  • Vector type
    Plant Expression
  • Promoter M24
  • Selectable markers
    Kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer NOS-F (5'-GCGTTCAAAAGTCGCCTAAG-3')
  • 3′ sequencing primer NOSterm-R (5'-attgccaaatgtttgaacga-3')
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

The difference between this plasmid and pKDH (Addgene plasmid # 67584) is that pSIM24 KanR has a kanamycin resistance cassette where pKDH has a hygromycin resistance cassette. The kanamycin and hygromycin resistance cassettes are used for selection in plants.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSIM24 KanR was a gift from Indu Maiti (Addgene plasmid # 67583 ; http://n2t.net/addgene:67583 ; RRID:Addgene_67583)
  • For your References section:

    pSiM24 is a novel versatile gene expression vector for transient assays as well as stable expression of foreign genes in plants. Sahoo DK, Dey N, Maiti IB. PLoS One. 2014 Jun 4;9(6):e98988. doi: 10.1371/journal.pone.0098988. eCollection 2014. PONE-D-14-03371 [pii] PubMed 24897541