Purpose(Empty Backbone) Gene expression in plants. A promoter and/or reporter can be cloned into this plasmid
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||67584||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerStratagene, fragments of the vector were synthesized by GenArt-Life Technologies
- Backbone size (bp) 6675
Vector typePlant Expression
- Promoter None
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer NOS-F (5'-GCGTTCAAAAGTCGCCTAAG-3')
- 3′ sequencing primer NOSterm-R (5'-attgccaaatgtttgaacga-3') (Common Sequencing Primers)
A reporter or gene of interest can be incorporated into pKDH at XhoI and SacI sites. A promoter can be incorporated into pKDH at EcoRI and HindIII sites.
pKDH contains a hygromycin resistance cassette.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pKDH was a gift from Indu Maiti (Addgene plasmid # 67584 ; http://n2t.net/addgene:67584 ; RRID:Addgene_67584)
For your References section:pSiM24 is a novel versatile gene expression vector for transient assays as well as stable expression of foreign genes in plants. Sahoo DK, Dey N, Maiti IB. PLoS One. 2014 Jun 4;9(6):e98988. doi: 10.1371/journal.pone.0098988. eCollection 2014. PONE-D-14-03371 [pii] PubMed 24897541