Purpose(Empty Backbone) Derivative of pSiM24 plant expression vector without the M24 promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||67884||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerStratagene, fragments of the vector were synthesized by GenArt-Life Technologies
- Backbone size (bp) 6555
Vector typePlant Expression
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer NOS-F (5'-GCGTTCAAAAGTCGCCTAAG-3')
- 3′ sequencing primer NOSterm-R (5'-attgccaaatgtttgaacga-3') (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
The kanamycin resistance cassette is used for selection in plants.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSi was a gift from Indu Maiti (Addgene plasmid # 67884 ; http://n2t.net/addgene:67884 ; RRID:Addgene_67884)
For your References section:pSiM24 is a novel versatile gene expression vector for transient assays as well as stable expression of foreign genes in plants. Sahoo DK, Dey N, Maiti IB. PLoS One. 2014 Jun 4;9(6):e98988. doi: 10.1371/journal.pone.0098988. eCollection 2014. PONE-D-14-03371 [pii] PubMed 24897541