Optimization of Insect Odorant Receptor Trafficking and Functional Expression Via Transient Transfection in HEK293 Cells.
Miazzi F, Hoyer C, Sachse S, Knaden M, Wicher D, Hansson BS, Lavista-Llanos S
Chem Senses. 2019 Oct 26;44(9):673-682. doi: 10.1093/chemse/bjz062. PubMed Article
Miazzi F, Hoyer C, Sachse S, Knaden M, Wicher D, Hansson BS, Lavista-Llanos S
Chem Senses. 2019 Oct 26;44(9):673-682. doi: 10.1093/chemse/bjz062. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 126472 | pcDNA3.1(-)-mRho.V5.mER.hOr47a | Human codon-optimized version of D. melanogaster Or47a (hOr47a) with N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved intracellular trafficking in mammalian cells |
| 126473 | pcDNA3.1(-)-V5.mER.hOr47a | Human codon-optimized version of D. melanogaster Or47a (hOr47a) with an N-terminal tag derived from human HCN1 (mER) for improved intracellular trafficking in mammalian cells |
| 126474 | pcDNA3.1(-)-V5.hOr47a | Human codon-optimized version of D. melanogaster Or47a (hOr47a) without N-terminal tags for improved intracellular trafficking in mammalian cells |
| 126475 | pCMV-BI | High-copy number vector for the co-expression of two genes in mammalian cells |
| 126476 | pDmelOR | Vector for the functional expression of insect ORs in mammalian cells, with a human codon-optimized version of D.melanogaster Orco (hOrco) with an N-terminal myc tag and a β-globin/IgG chimeric intron |
| 126478 | pDmelOR-mRho.V5.mER.hOr47a | Human codon-optimized D. melanogaster Orco (hOrco) and Or47a (hOr47a). Or47a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells |
| 126479 | pDmelOR-mRho.V5.mER.hOr56a | Human codon-optimized D. melanogaster Orco (hOrco) and Or56a (hOr56a). Or56a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells |